Enabled (Xena) regulates neural plate morphogenesis, apical constriction, and cellular adhesion required for neural tube closure in Xenopus.

Developmental Biology
Julaine Roffers-AgarwalJeffrey R Miller

Abstract

Regulation of cellular adhesion and cytoskeletal dynamics is essential for neurulation, though it remains unclear how these two processes are coordinated. Members of the Ena/VASP family of proteins are localized to sites of cellular adhesion and actin dynamics and lack of two family members, Mena and VASP, in mice results in failure of neural tube closure. The precise mechanism by which Ena/VASP proteins regulate this process, however, is not understood. In this report, we show that Xenopus Ena (Xena) is localized to apical adhesive junctions of neuroepithelial cells during neurulation and that Xena knockdown disrupts cell behaviors integral to neural tube closure. Changes in the shape of the neural plate as well as apical constriction within the neural plate are perturbed in Xena knockdown embryos. Additionally, we demonstrate that Xena is essential for cell-cell adhesion. These results demonstrate that Xena plays an integral role in coordinating the regulation of cytoskeletal dynamics and cellular adhesion during neurulation in Xenopus.

References

Dec 24, 1992·The New England Journal of Medicine·A E Czeizel, I Dudás
Jan 1, 1991·Methods in Cell Biology·R M Harland
Jan 1, 1990·Progress in Neurobiology·A J CoppD L Cockroft
Jan 1, 1988·The Anatomical Record·G C SchoenwolfA Moe
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·D J StumpoP J Blackshear
Jan 1, 1997·Developmental Biology·G L RadiceR O Hynes
Nov 19, 1997·Trends in Neurosciences·J L Smith, G C Schoenwolf
Mar 9, 1999·Neuron·L M LanierF B Gertler
Jul 9, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P Ybot-Gonzalez, A J Copp
Nov 13, 1999·Nature Cell Biology·K RottnerJ Wehland
Mar 23, 2000·Mechanisms of Development·L S Gammill, H Sive
May 29, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J F Colas, G C Schoenwolf
Jan 5, 2002·The Journal of Cell Biology·E E GrevengoedM Peifer
Feb 28, 2002·The EMBO Journal·Rachel S DarkenPaul A Wilson
Nov 8, 2002·Development·John B Wallingford, Richard M Harland
Sep 17, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·A Sheila MenziesFrank B Gertler
Jan 22, 2005·Gene Expression Patterns : GEP·Sarah J WannerJeffrey R Miller
Mar 9, 2005·The Journal of Cell Biology·Yoshihiko ShimizuShuh Narumiya
Mar 22, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jennifer B XanthosJeffrey R Miller
Apr 5, 2005·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·John B Wallingford
Nov 4, 2005·Histochemistry and Cell Biology·Annette B GallerMatthias Reinhard
Dec 24, 2005·Molecular Biology of the Cell·Jeanie A ScottAlpha S Yap
Jan 20, 2006·Development·Katherine A Kragtorp, Jeffrey R Miller
May 11, 2006·The Journal of Biological Chemistry·Megan L DietzJeffrey D Hildebrand
Jan 19, 2007·Nature Reviews. Genetics·Jessica R K Seifert, Marek Mlodzik

❮ Previous
Next ❯

Citations

Mar 15, 2016·Experimental Eye Research·Catherine ChengVelia M Fowler
Mar 11, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Laura Anne Lowery, Hazel Sive
Apr 25, 2012·Development, Growth & Differentiation·Makoto SuzukiNaoto Ueno
Nov 21, 2009·Journal of Cellular Physiology·Rebecca Bakkevig SperryMarc D H Hansen
Sep 16, 2009·Developmental Biology·Jacob M SawyerBob Goldstein
Jun 27, 2021·Seminars in Cell & Developmental Biology·Alphy John, Matteo Rauzi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Related Papers

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Jennifer B XanthosJeffrey R Miller
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
A Sheila MenziesFrank B Gertler
© 2021 Meta ULC. All rights reserved