Enabling multienzyme biocatalysis using nanoporous materials

Biotechnology and Bioengineering
Bilal El-ZahabPing Wang

Abstract

Multistep reactions catalyzed by a covalently immobilized enzyme-cofactor-enzyme system were achieved. Lactate dehydrogenase (LDH), glucose dehydrogenase (GDH), and cofactor NADH were incorporated into two porous silica glass supports. One of the glass supports had pores of 30 nm in diameter, while the other was of 100-nm pore size. Effective shuttling of the covalently bound NADH between LDH and GDH was achieved, such that regeneration cycles of NADH/NAD(+) were observed. The glass of 30-nm pore size afforded enzyme activities that were about twice those observed for the glass of 100-nm pore size, indicating the former provided better enzyme-cofactor integration. The effect of the size of spacers was also examined. The use of longer spacers increased the reaction rates by approximately 18 times as compared to those achieved with glutaraldehyde linkage. It appeared that the concave configuration of the nanopores played an important role in enabling the multistep reactions. The same multienzyme system immobilized on nonporous polystyrene particles of 500-nm diameter was only approximately 2% active as the glass-supported system. It is believed that the nanoporous structure of the glass supports enhances the molecular interaction...Continue Reading

Citations

Jun 25, 2008·Applied Biochemistry and Biotechnology·Ping Wang
Jan 24, 2013·Analytical Chemistry·Xiaoyi FengSongping Zhang
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Lorena Betancor, Heatherk Luckarift
Jun 5, 2012·Nanoscale Research Letters·Galina A PetkovaVladimír Král
Jul 16, 2014·Critical Reviews in Biotechnology·Madan L VermaColin J Barrow
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Oct 4, 2011·Biotechnology Advances·Shakeel Ahmed Ansari, Qayyum Husain
Nov 13, 2008·Journal of Biotechnology·Wenfang LiuPing Wang
Apr 27, 2007·Biotechnology Advances·Wenfang Liu, Ping Wang
Nov 7, 2006·Current Opinion in Biotechnology·Ping Wang
Aug 8, 2007·Biotechnology and Bioengineering·Bilal El-ZahabPing Wang
Jan 13, 2006·Biotechnology Advances·Jungbae KimPing Wang
Jun 16, 2011·Biotechnology Advances·Y-H Percival Zhang
Dec 17, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Makoto YoshimotoNaoki Tsubomura
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Dec 10, 2020·Nanoscale·Seong-Min JoKatharina Landfester
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Apr 13, 2021·ACS Applied Nano Materials·Xiaoting XueLei Ye
Jul 9, 2010·Analytical Chemistry·Yulia A SkvortsovaMaxwell L Geng

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