Enabling Sensitive Phenotypic Profiling of Cancer-Derived Small Extracellular Vesicles Using Surface-Enhanced Raman Spectroscopy Nanotags

ACS Sensors
Wei ZhangYuling Wang

Abstract

Circulating cancer-derived small extracellular vesicles (EVs) are nanoscale membranous vesicles shed from cancer cells that are released into surrounding body fluids. Small EVs contain biomolecules associated with cancer such as DNA and proteins for cell-to-cell communication. Therefore, small EVs have been regarded as important cancer biomarkers for liquid biopsy-based cancer diagnosis and drug treatment monitoring. However, because of the high heterogeneity and low level of small EVs in body fluids, there is a high demand for sensitive detection and characterization of such vesicles at a molecular level. In this study, we have developed a sensitive and effective approach to simultaneously profile multiple protein biomarkers expressed on cancer-derived small EVs using surface-enhanced Raman spectroscopy (SERS) nanotags in a single test, without complex isolation steps. Rapid and multiplexed phenotypic profiling of small EVs is achieved by mixing specific detection antibody-coated SERS nanotags, filtered conditioned EV-suspended medium (conditioned EVs), and capture antibody (CD63)-conjugated magnetic beads to form a sandwich immunoassay. As a proof-of-concept demonstration, we applied this approach to characterize pancreatic c...Continue Reading

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Citations

Oct 3, 2020·The Analyst·Gregory Q Wallace, Jean-François Masson
Sep 4, 2020·Biology·Simona Bernardi, Carolina Balbi
Sep 4, 2020·International Journal of Molecular Sciences·Ihor ArkhypovViktor Umansky
Aug 23, 2020·Materials·Jin-Ha ChoiJeong-Woo Choi
Jan 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ralph El HallalYuling Wang
Feb 24, 2021·ACS Sensors·Lanqun Mao
Jun 18, 2021·Nature Protocols·Guillermo Bordanaba-FloritJuan M Falcón-Pérez
Sep 1, 2021·Advanced Science·Li MinShutao Wang

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