Enamel Matrix Derivative Promotes Healing of a Surgical Wound in the Rat Oral Mucosa

Journal of Periodontology
Tal Maymon-GilM Weinreb

Abstract

Enamel matrix proteins (EMPs) play a role in enamel formation and the development of the periodontium. Sporadic clinical observations of periodontal regeneration treatments with enamel matrix derivative (EMD), a commercial formulation of EMPs, suggest that it also promotes post-surgical healing of soft tissues. In vitro studies showed that EMD stimulates various cellular effects, which could potentially enhance wound healing. This study examines the in vivo effects of EMD on healing of an oral mucosa surgical wound in rats. A bilateral oral mucosa wound was created via a crestal incision in the anterior edentulous maxilla of Sprague-Dawley rats. Full-thickness flaps were raised, and, after suturing, EMD was injected underneath the soft tissues on one side, whereas the EMD vehicle was injected in the contralateral side. Animals were sacrificed after 5 or 9 days, and the wound area was subjected to histologic and immunohistochemical analysis of the epithelial gap, number of macrophages, blood vessels, proliferating cells, and collagen content in the connective tissue (CT). Gene expression analysis was also conducted 2 days post-surgery. EMD had no effect on the epithelial gap of the wound. On both days 5 and 9, EMD treatment incr...Continue Reading

References

Jan 1, 1995·Archives of Oral Biology·S J BrookesW A Bonass
Mar 1, 1994·Journal of Structural Biology·A G FinchamH C Slavkin
Oct 6, 1997·Journal of Clinical Periodontology·L Hammarström
Oct 6, 1997·Journal of Clinical Periodontology·S GestreliusM Somerman
Feb 13, 2001·Journal of Clinical Periodontology·S P LyngstadaasS Gestrelius
Feb 24, 2001·Clinical Oral Investigations·S GestreliusL Hammarström
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 16, 2002·Journal of Clinical Periodontology·Jan L Wennström, Jan Lindhe
May 20, 2003·Journal of Periodontal Research·J C RinconP M Bartold
Jul 31, 2003·Journal of Clinical Periodontology·Kuo YuanMing T Lin
Feb 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ursula MirastschijskiMagnus S Agren
Apr 17, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Angelica WackenforsMalin Malmsjö
Nov 22, 2005·Lancet·Bruce L PihlstromNewell W Johnson
Aug 16, 2006·Clinical Oral Implants Research·Frank SchwarzJürgen Becker
Dec 26, 2006·Journal of Dental Research·E ZeldichM Weinreb
Jan 4, 2007·Journal of Periodontology·Scott R SchlueterDavid L Cochran
Aug 23, 2008·Journal of Dental Research·E ZeldichM Weinreb
Mar 27, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Gregory S Schultz, Annette Wysocki
Jun 11, 2009·Journal of Clinical Periodontology·Amir SavageIan Needleman
Jul 25, 2009·Orthodontics & Craniofacial Research·S P LyngstadaasJ E Reseland
Apr 8, 2010·Journal of Periodontology·Amy S KauvarDavid L Cochran
Jan 5, 2011·Journal of Clinical Periodontology·Daniel S ThomaDavid L Cochran
Apr 20, 2011·Periodontology 2000·Yuichi IzumiIsao Ishikawa
Nov 11, 2011·Tissue Engineering. Part B, Reviews·H Michelle GrandinMichel Dard
Mar 30, 2013·Orthodontic Waves : Official Journal of the Japanese Orthodontic Society·Keigo Yoshizaki, Yoshihiko Yamada
Nov 25, 2014·Journal of Periodontal Research·R J MironM Weinreb
Apr 14, 2015·Periodontology 2000·Miron Weinreb, Carlos E Nemcovsky

❮ Previous
Next ❯

Citations

Mar 19, 2016·Journal of Clinical Periodontology·Richard J MironDieter D Bosshardt
Sep 4, 2019·Journal of Periodontology·Lorenzo TavelliWilliam V Giannobile
Jan 27, 2017·Experimental and Therapeutic Medicine·Marzena Wyganowska-SwiatkowskaMalgorzata Kotwicka
Sep 21, 2021·Journal of Periodontal Research·Manuela Maria Viana MiguelMauro Pedrine Santamaria

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.