Enantiomer separation of amino acids in immunoaffinity micro LC-MS

Chirality
Jessica M ZelekeOliver Hofstetter

Abstract

Chiral immunoaffinity microbore columns were directly interfaced with MS detection, and the effect of column length and temperature on the enantiomer separation of a number of underivatized aromatic and aliphatic amino acids was investigated utilizing an antibody chiral stationary phase that had been prepared by immobilizing a monoclonal anti-D-amino acid antibody onto silica. The stronger affinity of the antibody towards aromatic and bulky amino acids allowed separation of such analytes in a 0.75 x 150 mm column, while an increase in column length enabled separation of more weakly bound compounds. The strength of interaction between chiral selector and analytes could be modulated conveniently by lowering the temperature. For the first time, simultaneous enantiomer separation of mixtures of amino acids was achieved on antibody-based chiral stationary phases using extracted ion chromatograms.

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Citations

Jul 6, 2013·Protein Expression and Purification·Pierre P ElenisteOliver Hofstetter
Mar 3, 2009·Journal of Pharmaceutical and Biomedical Analysis·Elliott J FrancoOliver Hofstetter
Nov 21, 2007·Journal of Pharmaceutical and Biomedical Analysis·Elliott J FrancoOliver Hofstetter
Sep 18, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michael C Peoples, H Thomas Karnes
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Nov 18, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Volker Schurig
Mar 28, 2017·Separations·Cong BiDavid S Hage
May 17, 2008·Analytical Chemistry·Timothy J Ward, Beth Ann Baker

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