Enantiomeric separation of some demethylated analogues of clofibric acid by capillary zone electrophoresis and nano-liquid chromatography

Electrophoresis
Marialuigia FantacuzziS Fanali

Abstract

The enantiomeric separation of some demethylated analogues of clofibric acid, namely 2-(6-chloro-benzothiazol-2-ylsulfanyl)-, 2-(6-methoxy-benzothiazol-2-ylsulfanyl)-, 2-(quinolin-2-yloxy)-, 2-(6-chloro-quinolin-2-yloxy)-, 2-(7-chloro-quinolin-4-yloxy)-propionic acid (compounds A-E, respectively), has been studied by CZE and nano-LC using for the first technique two beta-CD derivatives and vancomycin added to the BGE and vancomycin-modified silica particles for the second one, with the aim to find the optimum experimental conditions for the baseline resolution. The type and the concentration of the chiral selector added to the BGE, the buffer pH, the type of organic modifier and its concentration, the capillary temperature and the applied voltage played a very important role in the enantioresolution of the analysed compounds. The use of 6-monodeoxy-6-monoamino-beta-CD allowed to achieve baseline resolution of four of five clofibric acid derivatives in less than 10 min while heptakis-(2,3,6-tri-O-methyl)-beta-CD partially resolved the same compounds in their enantiomers. Employing vancomycin as the chiral selector in CZE, the counter-current partial filling method was chosen achieving baseline resolution of four analytes. All th...Continue Reading

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Citations

Aug 3, 2010·Journal of Pharmaceutical and Biomedical Analysis·Aleksandra F ProkhorovaOleg A Shpigun
Aug 19, 2008·Journal of Chromatography. a·Gerald Gübitz, Martin G Schmid
Jul 12, 2007·Journal of Separation Science·Javier Hernández-BorgesSalvatore Fanali
Sep 7, 2007·Electrophoresis·Christian W Klampfl
Jan 8, 2013·Journal of Separation Science·Anna RoccoSalvatore Fanali
Sep 10, 2011·Electrophoresis·Aleksandra F ProkhorovaOleg A Shpigun
Oct 22, 2016·Journal of Chromatography. a·Salvatore Fanali

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