Enantiomeric separation using an l-RNA aptamer as chiral additive in partial-filling capillary electrophoresis

Analytical Chemistry
Josephine RutaEric Peyrin

Abstract

In this paper, we report the chiral resolution of arginine using an anti-arginine l-RNA aptamer chiral selector in partial-filling CE. The effects of the capillary temperature, sample load, and aptamer plug length on the enantiomeric separation were assessed. Very high chiral resolving capability was observed at low or moderate capillary temperatures (the target peak being not detected in the separation window), whereas the practical chiral resolution was achieved only at high enough temperatures (50-60 degrees C). Over this high-temperature range, the electrophoretic behavior of the target enantiomer appeared to result from a combination of binding site heterogeneity, slow desorption kinetics, and concentration overload of aptamer binding sites. From additional thermal UV melting experiments, three RNA conformations were identified for the 50-60 degrees C temperatures. It was suggested that the presence of these different RNA conformations was a plausible source of the binding site heterogeneity.

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Citations

Sep 28, 2007·Analytical and Bioanalytical Chemistry·Joséphine RutaEric Peyrin
Oct 7, 2011·Analytical and Bioanalytical Chemistry·Anthony KiddEric Peyrin
Mar 1, 2012·Analytical and Bioanalytical Chemistry·Kaiguang YangYukui Zhang
Nov 16, 2007·Chemical Communications : Chem Comm·Daisuke MiyamotoMizuo Maeda
Oct 14, 2010·RNA·Mali IllangasekareMichael Yarus
Mar 5, 2016·Analytical and Bioanalytical Chemistry·Kai LingFu-Rong Li
Mar 24, 2017·Life·Michael Yarus
May 17, 2007·Chemistry : a European Journal·Tao LiShaojun Dong
Nov 28, 2007·Electrophoresis·Eun Joo SongYoung Sook Yoo
Oct 24, 2009·Physical Chemistry Chemical Physics : PCCP·Po-Hsun LinWen-Yih Chen
Mar 27, 2019·Chemistry : a European Journal·Brian E YoungJonathan T Sczepanski
Jan 25, 2020·Electrophoresis·Hana NevídalováZdeněk Glatz
Feb 4, 2011·Journal of the American Chemical Society·Thi-Huong NguyenRüdiger Berger
May 22, 2007·Analytical Chemistry·Josephine RutaEric Peyrin

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