Enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile for production of (R)-(-)-mandelic acid by a newly isolated mutant strain.

Journal of Industrial Microbiology & Biotechnology
Ya-Ping XueYin-Chu Shen

Abstract

(R)-(-)-Mandelic acid (R-MA) is an important intermediate with broad uses. Recently, R-MA production using nitrilase has been gaining more and more attention due to its higher productivity and enantioselectivity. In this work, a new bacterium WT10, which exhibited favorable nitrilase activity and excellent enantioselectivity for production of R-MA by enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile, was isolated and identified as a strain of Alcaligenes faecalis. In order to improve its nitrilase activity for industrial application, the wild-type strain WT10 was further subjected to mutagenesis using a combined LiCl-ultraviolet irradiation and low energy N(+) ion beams implantation technique. A valuable mutant strain A. faecalis ZJUTB10 was obtained. The nitrilase specific activity of the mutant strain was greatly improved up to 350.8 U g(-1), in comparison with wild-type strain WT10 of 53.09 U g(-1). The reaction conditions for R-MA production by mutant strain A. faecalis ZJUTB10 were also optimized. Nitrilase activity in mutant strain showed a broad pH optimum at pH 7.7-8.5. The optimal temperature was 35°C. The highest production rate reached 9.3 mmol h(-1) g(-1). The results showed that mutant strain A. faec...Continue Reading

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Citations

Oct 31, 2012·Microbial Cell Factories·Jin-Song GongZheng-Hong Xu
Aug 3, 2014·Journal of Industrial Microbiology & Biotechnology·Li-Qun JinYin-Chu Shen
Oct 23, 2012·World Journal of Microbiology & Biotechnology·Li-Qun JinYu-Guo Zheng
Jun 27, 2013·Applied Microbiology and Biotechnology·Jin-Song GongZheng-Hong Xu
Nov 16, 2013·Applied Microbiology and Biotechnology·Zhong-Yu YouYu-Guo Zheng
Feb 20, 2016·Applied Microbiology and Biotechnology·Ya-Ping XueYu-Guo Zheng
Jul 6, 2013·Biotechnology and Applied Biochemistry·Pramod W RamtekeBharat J Wadher
Oct 14, 2020·Critical Reviews in Biotechnology·Ji-Dong ShenYu-Guo Zheng
Sep 15, 2011·Journal of Agricultural and Food Chemistry·Zhi-Qiang LiuYin-Chu Shen

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