Enantioselective Dechlorination of Polychlorinated Biphenyls in Dehalococcoides mccartyi CG1

Applied and Environmental Microbiology
Ling YuBixian Mai

Abstract

Reductive dehalogenation mediated by organohalide-respiring bacteria plays a critical role in the global cycling of organohalides. Nonetheless, information on the dehalogenation enantioselectivity of organohalide-respiring bacteria remains limited. In this study, we report the enantioselective dechlorination of chiral polychlorinated biphenyls (PCBs) by Dehalococcoides mccartyi CG1. CG1 preferentially removed halogens from the (-)-enantiomers of the three major environmentally relevant chiral PCBs (PCB174, PCB149, and PCB132), and the enantiomer compositions of the dechlorination products depended on their parent organohalides. The in vitro assays with crude cell extracts or concentrated whole cells and the in vivo experiments with living cells showed similar enantioselectivities, in contrast with the distinct enantiomeric enrichment factors (εER) of the substrate chiral PCBs. Additionally, these results suggest that concentrated whole cells might be an alternative to crude cell extracts in in vitro tests of reductive dehalogenation activities. The enantioselective dechlorination of other chiral PCBs that we resolved via gas chromatography further confirmed the preference of CG1 for the (-)-enantiomers.IMPORTANCE A variety of a...Continue Reading

References

Sep 1, 1992·Microbiological Reviews·W W Mohn, J M Tiedje
May 16, 2001·Environmental Science & Technology·C S WongW T Foreman
Oct 7, 2004·Applied and Environmental Microbiology·Tina A MüllerHans-Peter E Kohler
Oct 21, 2004·Science in China. Series C, Life Sciences·Qixing ZhouJidong Liang
Nov 2, 2005·Methods in Enzymology·Frank E LöfflerKirsti M Ritalahti
May 30, 2006·Protein Science : a Publication of the Protein Society·Tina A MüllerRobert P Hausinger
Aug 23, 2007·Environmental Science & Technology·Perihan Binnur Kurt-KarakusKevin C Jones
Aug 24, 2007·Environmental Toxicology and Chemistry·Charles S WongDerek C G Muir
Jan 29, 2008·Applied and Environmental Microbiology·Harold D MayKevin R Sowers
Apr 14, 2010·Environmental Science & Technology·Hans-Joachim LehmlerCharles S Wong
Feb 22, 2012·Reviews of Environmental Contamination and Toxicology·Elin M UlrichWilliam T Foreman
Aug 25, 2012·The Journal of Physical Chemistry. a·Mitsunobu TodaTadashi Mori
Mar 16, 2013·Current Opinion in Biotechnology·Ruth Ellen Richardson
Jul 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shanquan WangJianzhong He
Oct 4, 2014·Science·Martin BommerHolger Dobbek
Jan 17, 2016·Current Opinion in Biotechnology·Ivonne Nijenhuis, Kevin Kuntze
Mar 15, 2016·Frontiers in Microbiology·Bat-Erdene JugderMichael Manefield
Apr 10, 2018·Biotechnology Advances·Shanquan WangRongliang Qiu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.