Enantioselective determination of (R)- and (S)-lansoprazole in human plasma by chiral liquid chromatography with mass spectrometry and its application to a stereoselective pharmacokinetic study

Journal of Separation Science
Luning SunYongqing Wang

Abstract

A simple and enantioselective method was developed and validated for the simultaneous determination of (R)- and (S)-lansoprazole in human plasma by chiral liquid chromatography with tandem mass spectrometry. Lansoprazole enantiomers and internal standard (esomeprazole) were extracted from plasma using acetonitrile as protein precipitating agent. Baseline chiral separation was achieved within 9.0 min on a Chiralpak IC column (150 mm × 4.6 mm, 5 μm) with the column temperature of 30°C. The mobile phase consisted of 10 mM ammonium acetate solution containing 0.05% acetic acid/acetonitrile (50:50, v/v). The mass spectrometric analysis was performed using a QTrap 5500 mass spectrometer coupled with an electrospray ionization source in positive ion mode. The multiple reactions monitoring transitions of m/z 370.1→252.1 and 346.1→198.1 were used to quantify lansoprazole enantiomers and esomeprazole, respectively. For each enantiomer, no apparent matrix effect was found, the calibration curve was linear over 5.00-3000 ng/mL, the intra- and inter-day precisions were below 10.0%, and the accuracy was -3.8 to 3.3%. Analytes were stable during the study. No chiral inversion was observed during sample storage, preparation procedure and analy...Continue Reading

References

Nov 1, 1993·British Journal of Clinical Pharmacology·B FlouvatF Dellatolas
Apr 15, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Masatomo MiuraToshio Suzuki
Mar 4, 2011·Annals of Medicine·Tiberiu HershcoviciRonnie Fass

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Citations

Feb 1, 2017·European Journal of Clinical Pharmacology·Yue-Qi LiYong-Qing Wang
Apr 16, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Muhammad Shoaib KhanMohammed Yaqoob
Aug 3, 2017·Archives of Pharmacal Research·Hyun Kyu ChungKyeong Ho Kim

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