Encapsidated Host Factors in Alphavirus Particles Influence Midgut Infection of Aedes aegypti

Viruses
David Mackenzie-LiuRichard W Hardy

Abstract

Transmission of mosquito-borne viruses requires the efficient infection of both a permissive vertebrate host and a competent mosquito vector. The infectivity of Sindbis virus (SINV), the type species of the Alphavirus genus, is influenced by both the original and new host cell. We have shown that infection of vertebrate cells by SINV, chikungunya virus (CHIKV), and Ross River virus (RRV) produces two subpopulations of virus particles separable based on density. In contrast, a single population of viral particles is produced by mosquito cells. Previous studies demonstrated that the denser vertebrate-derived particles and the mosquito-derived particles contain components of the small subunit of the host cell ribosome, whereas the less dense vertebrate-derived particles do not. Infection of mice with RRV showed that both particle subpopulations are produced in an infected vertebrate, but in a tissue specific manner with serum containing only the less dense version of the virus particles. Previous infectivity studies using SINV particles have shown that the denser particles (SINVHeavy) and mosquito derived particles SINVC6/36 are significantly more infectious in vertebrate cells than the less dense vertebrate derived particles (SIN...Continue Reading

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Citations

Sep 15, 2020·Seminars in Cell & Developmental Biology·Kate DickerAlfredo Castello

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Methods Mentioned

BETA
glycosylation
PCR
fluorescence microscopy

Software Mentioned

NanoJect

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