Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV CP.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Ana Luisa Duran-MezaJaime Ruiz-Garcia

Abstract

Different types of gold nanoparticles have been synthesized that show great potential in medical applications such as medical imaging, bio-analytical sensing and photothermal cancer therapy. However, their stability, polydispersity and biocompatibility are major issues of concern. For example, the synthesis of gold nanorods, obtained through the elongated micelle process, produce them with a high positive surface charge that is cytotoxic, while gold nanoshells are unstable and break down in a few weeks due to the Ostwald ripening process. In this work, we report the self-assembly of the capsid protein (CP) of cowpea chlorotic mottle virus (CCMV) around spherical gold nanoparticles, gold nanorods and gold nanoshells to form virus-like particles (VLPs). All gold nanoparticles were synthesized or treated to give them a negative surface charge, so they can interact with the positive N-terminus of the CP leading to the formation of the VLPs. To induce the protein self-assembly around the negative gold nanoparticles, we use different pH and ionic strength conditions determined from a CP phase diagram. The encapsidation with the viral CP will provide the nanoparticles better biocompatibility, stability, monodispersity and a new biolog...Continue Reading

References

Apr 6, 2001·Physical Review Letters·B WangJ Zhao
Jun 6, 2003·Journal of the American Chemical Society·Bogdan DragneaC Cheng Kao
Mar 25, 2004·Journal of the American Chemical Society·Yugang Sun, Younan Xia
Nov 16, 2004·Journal of Virological Methods·Jean-Philippe MichelLaurence Lavelle
Dec 17, 2004·Physical Review Letters·R T SengerS Ciraci
Feb 9, 2006·Journal of the American Chemical Society·Xiaohua HuangMostafa A El-Sayed
Apr 6, 2006·Journal of the American Chemical Society·Lina LooStefan Franzen
Apr 13, 2006·Nano Letters·Chao ChenBogdan Dragnea
Aug 11, 2006·The Journal of Physical Chemistry. B·J KimlingA Plech
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jingchuan SunBogdan Dragnea
Sep 7, 2007·Journal of Virological Methods·Laurence LavelleMari Gingery
Mar 21, 2008·Chemical Communications : Chem Comm·Andrés de la EscosuraJeroen J L M Cornelissen
Jul 4, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Rajesh SardarRoyce W Murray
Aug 13, 2009·The Journal of Physical Chemistry. B·L LavelleJ Ruiz-Garcia
Dec 7, 2010·Nanoscale·Craig JolleyTrevor Douglas
Dec 18, 2010·Trends in Microbiology·Adam Zlotnick, Suchetana Mukhopadhyay
May 20, 2011·Current Opinion in Biotechnology·Ibrahim YildizNicole F Steinmetz
Jul 2, 2011·Journal of Colloid and Interface Science·Sankar Kalidas SivaramanVenugopal Santhanam
Jul 19, 2011·ACS Applied Materials & Interfaces·Varadee VongsavatT Randall Lee
Oct 20, 2011·The British Journal of Radiology·S JainJ M O'Sullivan
Nov 13, 2012·International Journal of Nanomedicine·Eleonore Fröhlich
Jan 1, 2008·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Christopher G Khoury, Tuan Vo-Dinh
Jan 29, 2014·The Journal of Physical Chemistry. B·J R Vega-AcostaJ Ruiz-García
Feb 15, 2014·Biomedical Materials·Zhongshi LiangShunying Liu
May 20, 2014·Enzyme and Microbial Technology·Lorena Sánchez-SánchezRafael Vazquez-Duhalt
May 27, 2014·Nanoscale·Lise Schoonen, Jan C M van Hest
May 21, 2015·Biophysical Journal·Bodo D WiltsChristoph F Schmidt
May 3, 2016·The Journal of Physical Chemistry. B·Aijie LiuJeroen J L M Cornelissen
Apr 21, 2018·Talanta·Narges ElahiMohammad Hadi Baghersad
Apr 27, 2019·Frontiers in Chemistry·Jeremy B VinesHansoo Park
Nov 30, 2019·BioMed Research International·María V Villagrana-EscareñoJaime Ruíz-García

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Citations

Jan 26, 2021·Beilstein Journal of Organic Chemistry·Brandon Charles Seychell, Tobias Beck
Sep 11, 2021·PloS One·A L Duran-MezaW M Gelbart

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Methods Mentioned

BETA
transmission electron microscopy
dynamic light scattering
electrophoresis

Software Mentioned

ImageJ

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