Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams

PloS One
Ana Patrícia C SilvaRenato L Santos

Abstract

This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.

References

Jul 1, 1987·Journal of Biomaterials Applications·G E VisscherG J Argentieri
Aug 1, 1997·Preventive Veterinary Medicine·J M Blasco
Jun 6, 2000·Advanced Drug Delivery Reviews·R LangerJ Hanes
Jul 8, 2000·Biologicals : Journal of the International Association of Biological Standardization·M J GrillóJ M Blasco
Feb 21, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mitsuko TakenagaRie Igarashi
Nov 5, 2002·Veterinary Microbiology·Klaus Nielsen
Nov 5, 2002·Veterinary Microbiology·Gerhardt G SchurigMichael J Corbel
Nov 5, 2002·Veterinary Microbiology·D S Davis, P H Elzer
Nov 30, 2007·The Lancet Infectious Diseases·María Pía FrancoHenk L Smits
Feb 11, 2009·Journal of Wildlife Diseases·Angela M Arenas-GamboaAllison C Rice-Ficht
Feb 9, 2011·Infection and Immunity·Teane M A SilvaRenato L Santos
Jul 11, 2013·Revue Scientifique Et Technique·F P PoesterR L Santos
Jul 11, 2013·Revue Scientifique Et Technique·S C Olsen
Mar 5, 2014·TheScientificWorldJournal·Valéria S MoustacasTatiane A Paixão
Jul 18, 2014·Veterinary Research·Pedro Soler-LlorénsIgnacio López-Goñi
Apr 14, 2015·Vaccine·Renata Chagas BastosIvna Alana Freitas Brasileiro da Silveira

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Citations

Aug 19, 2016·PLoS Neglected Tropical Diseases·Aseem PandeyPaul de Figueiredo
Nov 16, 2016·PloS One·Tatiane F CarvalhoRenato L Santos
Nov 16, 2019·Veterinary Research·Beatriz Aragón-ArandaRaquel Conde-Álvarez
Sep 25, 2020·Innate Immunity·Zhixiong ZhouHanwei Jiao
Oct 9, 2018·Frontiers in Microbiology·Rebeca Singh Sidhu-MuñozNieves Vizcaíno
Sep 24, 2020·Microbiology and Immunology·Luciana Fachini CostaRenato Lima Santos
Feb 14, 2021·International Journal of Biological Macromolecules·Cláudia E V RochaTatiane A Paixão
Mar 20, 2019·Veterinary Immunology and Immunopathology·Alejandra Graciela DíazSilvia Marcela Estein
Oct 16, 2020·Microbiology and Molecular Biology Reviews : MMBR·Edgardo Moreno, Elías Barquero-Calvo
Aug 3, 2021·Frontiers in Microbiology·Lauren W Stranahan, Angela M Arenas-Gamboa

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Methods Mentioned

BETA
light
scanning electron microscopy
fluorescence microscopy
PCR
light microscopy

Software Mentioned

GraphPad Prisma

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