PMID: 11933216Apr 5, 2002Paper

Encapsulated cells producing retroviral vectors for in vivo gene transfer

The Journal of Gene Medicine
R SallerW H Günzburg

Abstract

Because gene therapy of the future will primarily take an in vivo approach, a number of problems associated with its current implementation exist. Currently, repeated delivery of a vector in vivo is necessary to ensure adequate transfer of the therapeutic gene. This may lead to the development of an immune response against the vector, thus interfering with gene delivery. To circumvent this problem, retroviral vector packaging cells that permanently produce recombinant retroviral vector particles have been encapsulated. Vector (pBAG)-producing amphotropic cells were encapsulated in beads composed of polymerized cellulose sulphate. These capsules were analysed in vitro for expression of the vector construct using X-gal staining, as well as for the release of particles by performing RT-PCR from culture supernatant. Infectivity studies were performed in vitro and in vivo. The latter was assayed using histological sections of the microcapsule and the surrounding area stained for beta-galactosidase activity and by RT-PCR. In culture, the virus-producing cells inside the capsules remained viable and released virus into the culture medium for at least 6 weeks. To test whether these capsules, upon implantation into mice, also release ve...Continue Reading

Citations

Feb 5, 2005·Cancer Immunology, Immunotherapy : CII·Peter DallDieter Niederacher
Feb 6, 2004·Trends in Biotechnology·Gorka OriveJosé Luis Pedraz
Aug 6, 2003·Trends in Molecular Medicine·Walter H Günzburg
Jul 23, 1999·Annals of the New York Academy of Sciences·J C KrögerM Löhr
May 9, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mona Dvir-GinzbergRiad Agbaria

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