Encapsulation and Controlled Release of Protein Guests by the Bacillus subtilis Lumazine Synthase Capsid

Biochemistry
Xue Han, Kenneth J Woycechowsky

Abstract

In Bacillus subtilis, the 60-subunit dodecahedral capsid formed by lumazine synthase (BsLS) acts as a container for trimeric riboflavin synthase (BsRS). To test whether the C-terminal sequence of BsRS is responsible for its encapsulation by BsLS, the green fluorescent protein (GFP) was fused to either the last 11 or the last 32 amino acids of BsRS, yielding variant GFP11 or GFP32, respectively. After purification, BsLS capsids that had been co-produced in bacteria with GFP11 and GFP32 are 15- and 6-fold more fluorescent, respectively, than BsLS co-produced with GFP lacking any BsRS fragment, indicating complex formation. Enzyme-linked immunosorbent assay experiments confirm that GFP11 is localized within the BsLS capsid. In addition, fusing the last 11 amino acids of BsRS to the C-terminus of the Abrin A chain also led to its encapsulation by BsLS at a level similar to that of GFP11. Together, these results demonstrate that the C-terminal tail of BsRS can act as an encapsulation tag capable of targeting other proteins to the BsLS capsid interior. As with the natural BsLS-BsRS complex, mild changes in pH and buffer identity trigger dissociation of the GFP11 guest, accompanied by a substantial expansion of the BsLS capsid. This s...Continue Reading

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Citations

May 2, 2018·Chemical Society Reviews·Yusuke AzumaDonald Hilvert
Dec 12, 2020·Frontiers in Bioengineering and Biotechnology·Liudmila A AverianovaLiudmila A Tekutyeva
Jan 16, 2021·Chembiochem : a European Journal of Chemical Biology·Roy A J F OerlemansJan C M van Hest
Apr 3, 2020·Biochemistry·Jiannan Fu, Kenneth J Woycechowsky
Jun 13, 2018·Bioconjugate Chemistry·Shuxin WangKenneth J Woycechowsky
Sep 10, 2021·Biomacromolecules·Antonina NaskalskaJonathan G Heddle

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