Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

The Analyst
G FaccioM Richter

Abstract

Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium-silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.

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Citations

May 8, 2018·Sensors·Longyi ChenJin Zhang
Aug 29, 2017·ACS Applied Materials & Interfaces·Leyla Nesrin KahyaogluJenna L Rickus
Aug 22, 2021·Biotechnology Advances·Hèctor López-LagunaUgutz Unzueta

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