Encapsulation of Lactobacillus plantarum 423 and its Bacteriocin in Nanofibers

Probiotics and Antimicrobial Proteins
Tiaan HeunisLeon M T Dicks

Abstract

Plantaricin 423, produced by Lactobacillus plantarum 423, was encapsulated in nanofibers that were produced by the electrospinning of 18% (w/v) polyethylene oxide (200 000 Da). The average diameter of the nanofibers was 288 nm. Plantaricin 423 activity decreased from 51 200 AU/ml to 25 600 AU/ml and from 204 800 AU/ml to 51 200 AU/ml after electrospinning, as determined against Lactobacillus sakei DSM 20017 and Enterococcus faecium HKLHS, respectively. Cells of L. plantarum 423 encapsulated in nanofibers decreased from 2.3 × 10(10) cfu/ml before electrospinning to 4.7 × 10(8) cfu/ml thereafter. Cells entrapped in the nanofibers continued to produce plantaricin 423. This is the first report on the encapsulation of a bacteriocin and cells of L. plantarum in nanofibers. The method may be used to design a drug delivery system for bacteriocins and the encapsulation of probiotic lactic acid bacteria. The technology is currently being optimized.

References

Sep 5, 1998·International Journal of Food Microbiology·I IvanovaP Boyaval
Jun 5, 1999·International Journal of Food Microbiology·C M FranzM E Stiles
Feb 13, 2001·Journal of Biotechnology·M SaarelaT Mattila-Sandholm
Nov 27, 2001·Science·J D HartgerinkS I Stupp
May 7, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·El-Refaie KenawyGary E Wnek
Dec 17, 2002·Journal of Industrial Microbiology & Biotechnology·A Caridi
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Apr 24, 2003·Antimicrobial Agents and Chemotherapy·John F Kokai-KunJames J Mond
Oct 22, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jing ZengXiabin Jing
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·R David PridmoreMark A Schell
Jul 30, 2004·The Journal of Antimicrobial Chemotherapy·Danuta KruszewskaAsa Ljungh
Jan 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Eric AltermannTodd R Klaenhammer
Jul 12, 2005·Biomacromolecules·Sing Yian ChewKam W Leong
Jul 20, 2005·Journal of Basic Microbiology·Svetoslav D Todorov, Leon M T Dicks
Sep 12, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yusuke NagaiShuguang Zhang
Sep 22, 2007·Advanced Drug Delivery Reviews·Dehai LiangBenjamin Chu
Mar 4, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sascha MaretschekThomas Kissel
Nov 21, 2008·Letters in Applied Microbiology·M De KwaadstenietL M T Dicks

❮ Previous
Next ❯

Citations

Aug 28, 2010·Journal of Biomedicine & Biotechnology·T D J Heunis, L M T Dicks
Dec 29, 2012·Beneficial Microbes·D Gawkowski, M L Chikindas
May 6, 2016·Current Microbiology·Jayesh J Ahire, Leon M T Dicks
Jun 30, 2011·Journal of Agricultural and Food Chemistry·Wai-Yee FungMin-Tze Liong
Oct 4, 2016·Frontiers in Microbiology·Hazem A FahimAhmed O El-Gendy
Jun 9, 2018·Critical Reviews in Food Science and Nutrition·Elham Assadpour, Seid Mahdi Jafari
Jun 5, 2013·Antimicrobial Agents and Chemotherapy·Tiaan D J HeunisLeon M T Dicks
Oct 19, 2011·Future Medicinal Chemistry·Osama BshenaBert Klumperman
Jul 25, 2019·Macromolecular Bioscience·Alexander DartPeter Kingshott
May 3, 2013·Probiotics and Antimicrobial Proteins·Nicolás I TorresMichael L Chikindas
Sep 26, 2020·Frontiers in Microbiology·Santosh Kumar TiwariMichael L Chikindas
Nov 29, 2016·Asian Pacific Journal of Tropical Medicine·Ameer KhusroPaul Agastian
Mar 12, 2020·Frontiers in Bioengineering and Biotechnology·Spase Stojanov, Aleš Berlec
Jul 14, 2020·Veterinary Research Communications·Laureano SchofsSergio F Sánchez Bruni
Feb 11, 2019·Biotechnology Letters·Gobinath ChandrakasanNorberto Chavarría-Hernández
Mar 30, 2016·Materials·Ji Wang, Wilfred Vermerris
Jul 1, 2019·Comprehensive Reviews in Food Science and Food Safety·María G Venegas-OrtegaGuadalupe V Nevárez-Moorillón
Jan 22, 2021·Journal, Genetic Engineering & Biotechnology·Sara Adel AmerMahmoud Ahmed Mailam
May 9, 2021·Applied and Environmental Microbiology·Anton Du Preez van StadenCarine Smith

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.