Encapsulation of Mesenchymal Stem Cells in 3D Ovarian Cell Constructs Promotes Stable and Long-Term Hormone Secretion with Improved Physiological Outcomes in a Syngeneic Rat Model.

Annals of Biomedical Engineering
Sivanandane SittadjodyEmmanuel C Opara

Abstract

Loss of ovarian function (e.g., due to menopause) leads to profound physiological effects in women including changes in sexual function and osteoporosis. Hormone therapies are a known solution, but their use has significantly decreased due to concerns over cardiovascular disease and certain cancers. We recently reported a tissue-engineering strategy for cell hormone therapy (cHT) in which granulosa cells and theca cells are encapsulated to mimic native ovarian follicles. cHT improved physiological outcomes and safety compared to pharmacological hormone therapies in a rat ovariectomy model. However, cHT did not achieve estrogen levels as high as ovary-intact animals. In this report, we examined if hormone secretion from cHT constructs is impacted by incorporation of bone marrow-derived mesenchymal stem cells (BMSC) since these cells contain regulatory factors such as aromatase necessary for estrogen production. Incorporation of BMSCs led to enhanced estrogen secretion in vitro. Moreover, cHT constructs with BMSCs achieved estrogen secretion levels significantly greater than constructs without BMSCs in ovariectomized rats from 70 to 90 days after implantation, while also regulating pituitary hormones. cHT constructs with BMSC ame...Continue Reading

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Citations

Apr 7, 2020·Nature Biomedical Engineering·Emma S GargusTeresa K Woodruff
Jul 25, 2020·Reproductive Biology and Endocrinology : RB&E·Ahmed Baker AlshaikhMats Hellström
Jan 30, 2021·Reproductive Sciences·Myung Jae JeonJohn D Jackson
Apr 21, 2021·Reproductive Sciences·Sivanandane SittadjodyJames J Yoo
Jun 8, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Du XiangYanfeng Wang
Aug 12, 2021·Annals of Biomedical Engineering·Raffaella De Vita, Jennifer Munson

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Methods Mentioned

BETA
fluorescence imaging
ELISAs
ELISA
blood draws

Software Mentioned

Pro Plus
Image J
SPSS
ImagePro Plus
pHT
Image

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