Encapsulation of OKT3 cells in hollow fibers

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
L H GranickaA Werynski

Abstract

Encapsulation of an OKT3 cell line in hollow fibers was evaluated in vitro and in vivo. The cell line is a mouse hybridoma producing immunoglobulin G2a (IgG2a) against CD3 human T lymphocytes and thus may function as a nonspecific activation system of a subpopulation of human T lymphocytes. For encapsulation purpose, hollow fibers of polypropylene K600 PP Accurel (Akzo, Germany) were selected. Hollow fibers were siliconized to improve membrane biocompatibility for in vivo experiments. The siliconized hollow fibers exhibited acceptable diffusive permeability (P) [ml/min/m2] for small solutes (for creatinine, p = 63.9 +/- 2.0, n = 3) and larger solutes (for albumin, p = 16.9 +/- 1.9, n = 3; for IgG, p = 1.0 +/- 0.2, n = 3). The 12 cm long hollow fibers were filled with a suspension of OKT3 cells of an average density of 10(6) cells/ ml, and both ends were sealed. The encapsulated cells were cultivated in RPMI 1640/10% CS medium at 37 degrees C, 5% CO2 for a period of 3 to 4 days. After the culture period, the medium was tested on human peripheral blood lymphocytes for the presence of anti-CD3 antibody and read in a flow FACS-trac cytometer (Becton Dickinson Immunocytochemistry Systems, San Diego, CA). The tightness of hollow fibe...Continue Reading

Citations

Aug 16, 2000·Advanced Drug Delivery Reviews·H UludagP A Tresco
Aug 16, 2000·Advanced Drug Delivery Reviews·B Ríhová
Nov 26, 2013·Advanced Drug Delivery Reviews·Paul de VosMarijke M Faas
Apr 25, 2009·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Roshni S PatelMichael J Lysaght
Jun 25, 2005·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·L H GranickaJ Kawiak
Aug 9, 2003·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Ludomira H GranickaAndrzej Weryński
May 1, 2021·Nanomaterials·Monika DrabikLudomira H Granicka

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