Encapsulation of Photosystem I in Organic Microparticles Increases Its Photochemical Activity and Stability for Ex Vivo Photocatalysis

ACS Sustainable Chemistry & Engineering
Arianna CherubinMassimiliano Perduca

Abstract

Photosystem I (PSI) is a pigment binding multisubunit protein complex involved in the light phase of photosynthesis, catalyzing a light-dependent electron transfer reaction from plastocyanin to ferredoxin. PSI is characterized by a photochemical efficiency close to one, suggesting its possible application in light-dependent redox reaction in an extracellular context. The stability of PSI complexes isolated from plant cells is however limited if not embedded in a protective environment. Here we show an innovative solution for exploiting the photochemical properties of PSI, by encapsulation of isolated PSI complexes in PLGA (poly lactic-co-glycolic acid) organic microparticles. These encapsulated PSI complexes were able to catalyze light-dependent redox reactions with electron acceptors and donors outside the PLGA microparticles. Moreover, PSI complexes encapsulated in PLGA microparticles were characterized by a higher photochemical activity and stability compared with PSI complexes in detergent solution, suggesting their possible application for ex vivo photocatalysis.

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Citations

Apr 4, 2021·Nanomaterials·Federica VurroMassimiliano Perduca
May 26, 2020·Trends in Biotechnology·Alexandra H Teodor, Barry D Bruce

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Methods Mentioned

BETA
AFM
dynamic light scattering
Fluorescence
time-resolved
fluorescence spectroscopy
the

Software Mentioned

DSCRun

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