Encephalitogenic T cells in the B10.PL model of experimental allergic encephalomyelitis (EAE) are of the Th-1 lymphokine subtype.

Cellular Immunology
D G AndoE E Sercarz

Abstract

T helper cells reactive to myelin basic protein are clearly implicated in the pathogenesis of murine EAE. We have developed a T cell line, BML-1 that (1) is reactive to the encephalitogenic amino terminal nonapeptide (1-9NAC) of MBP, (2) is I-Au restricted, and (3) induces relapsing EAE in B10.PL (H-2u) mice. Measurement of the lymphokine profile of BML-1 revealed secretion of IL-2, interferon-gamma and lymphotoxin but not IL-4. This profile is consistent with the Th1/DTH subtype. Coculture of BML-1 with MBP-primed B cells shows that BML-1 does not provide significant helper function in vitro. In addition, BML-1 secretion of interferon-gamma was found to inhibit LPS-induced anti-MBP antibody responses. This suggested that anti-MBP antibodies may not be necessary for induction of EAE. Sera from mice, in which severe disease was induced with the 1-9NAC peptide and Bordetella pertussis, showed no development of serum antibodies to MBP. These data show that MBP-reactive Th cells of the Th-1/DTH subtype can induce EAE and do not provide Th function for anti-MBP responses and that serum anti-MBP antibodies are not found in peptide 1-9NAC-induced disease. T cell lines specific for encephalitogenic epitopes and characterized for lympho...Continue Reading

Citations

Mar 27, 2001·European Journal of Immunology·M P RileyA J Caton
Jan 1, 1994·Annals of Neurology·H WekerleC Linington
Jan 1, 1994·Annals of Neurology·S L Hauser
Jan 1, 1996·Springer Seminars in Immunopathology·R Martin, H McFarland
Jan 1, 1996·Springer Seminars in Immunopathology·M Bradl
Sep 1, 1991·Neurochemical Research·D Baker, A N Davison
Aug 13, 2013·Acta Neuropathologica·Daniel E LowtherDaniel M Altmann
Mar 29, 2012·Journal of Clinical Immunology·Hideho Okada, Samia J Khoury
Apr 10, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Rhoanne C McPherson, Stephen M Anderton
Apr 9, 2002·Current Neurology and Neuroscience Reports·Rhonda R Voskuhl
Feb 9, 2012·Current Treatment Options in Cardiovascular Medicine·Claudiu I DiaconuAlexander Rae-Grant
Nov 28, 2008·Neuroscience Bulletin·Min-Fang GuoCun-Gen Ma
May 1, 1993·Trends in Pharmacological Sciences·F Powrie, R L Coffman
May 5, 1994·Regulatory Peptides·H OffnerA A Vandenbark
Jun 1, 1993·Immunology Today·F Powrie, R L Coffman
Dec 1, 1993·Current Opinion in Immunology·A O'Garra, K Murphy
Dec 1, 1999·Developmental and Comparative Immunology·F SinigagliaL Rogge
Jun 16, 2000·Journal of Neuroimmunology·F AloisiL Adorini
Jan 29, 1999·Research in Immunology·M K Racke, A E Lovett-Racke

❮ Previous
Next ❯

Related Concepts