Apr 27, 2020

Conservation and divergence of DNA replication control in Chlamydomonas reinhardtii

BioRxiv : the Preprint Server for Biology
A. E. IkuiFrederick R. Cross

Abstract

We recently isolated temperature-sensitive cell cycle mutants in Chlamydomonas reinhardtii for which the causative mutations were located in genes annotated for potential involvement in DNA replication. Chlamydomonas has a very long G1 period during which cells grow up to ~10-fold without division, followed by rapid cycles of DNA replication and mitosis (multiple fission). All of the candidate DNA replication mutants tested were defective in completion of the first round of DNA replication, and also failed to produce mitotic spindles. For a subset of the mutants, we rescued temperature-sensitive lethality with tagged transgenes and used the resulting strains to analyze abundance and localization control of the tagged protein. All of the DNA replication proteins tested were essentially undetectable until late G1, accumulated during the period of multiple fission and then were degraded as cells completed their terminal divisions. MCM4 and MCM6 were localized to the nucleus during the division cycle except for transient cytoplasmic localization during mitosis. CDC45 showed strict protein location to the nucleus and co-localized to spindles during mitosis. In contrast, CDC6 was detected in the nucleus only transiently during early ...Continue Reading

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Mentioned in this Paper

Short Interspersed Nucleotide Elements
Tuberculosis
Health Services
Cessation of Life
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Antiretroviral Therapy
HIV Infections
Persons
Objective (Goal)
Prophylactic Treatment

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