Endischemic oxygen persufflation to improve viability of marginally preserved donor livers

Transplant International : Official Journal of the European Society for Organ Transplantation
Thomas MinorA Paul

Abstract

The quality of cold-stored donor livers slowly declines beyond approximately 12 h, although these organs may still be used for clinical transplantation. The aim of the present study was to improve the energetic status and viability of long-term-preserved livers by short-term gaseous oxygen insufflation prior to implantation of the organ using a technique that has already been shown to promote aerobic energy metabolism during hypothermia. Livers from ten male Wistar rats were isolated, rinsed blood-free. Five livers (group 1) were stored for 48 h at 4 degrees C in UW preservation solution, and five livers (group 2) were isolated and stored in the same manner for 47 h, and were then, during the last 60 min of the preservation period, connected to a persufflation device and gaseous oxygen was introduced into the organ via the inferior caval vein, with the liver still immersed in cold UW solution. This technique of endischemic gaseous oxygenation resulted in a significant normalization of vascular resistance upon isolated reperfusion in vitro and a reduction in hepatic efflux of alanine aminotransferase as well as glutamate dehydrogenase, which led to improved recovery of the reperfused grafts of group 2 as evidenced by an elevated...Continue Reading

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Citations

Feb 20, 2008·Langenbeck's Archives of Surgery·Steffen ManekellerAndreas Hirner
Apr 23, 2014·Nature Reviews. Gastroenterology & Hepatology·Maxim NebrigAndreas Pascher
Feb 4, 2012·Cryobiology·Thomas M SuszynskiKlearchos K Papas
Apr 27, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T MinorB Lüer
Sep 13, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T MinorA Paul
Apr 29, 2014·Journal of Hepatology·Jay A Graham, James V Guarrera
Oct 7, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Babak BananWilliam Chapman
Jul 29, 2003·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Antonino CavallariDavide D'amico
Feb 29, 2008·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Jürgen TreckmannAndreas Paul
Feb 4, 2010·Current Opinion in Organ Transplantation·Diethard Monbaliu, John Brassil
Apr 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P DutkowskiP A Clavien
Jul 11, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Steffen Manekeller, Thomas Minor

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