Endo-β-1,4-glucanases impact plant cell wall development by influencing cellulose crystallization

Journal of Integrative Plant Biology
Magdalena GlassShawn D Mansfield

Abstract

Cell walls are vital to the normal growth and development of plants as they protect the protoplast and provide rigidity to the stem. Here, two poplar and Arabidopsis orthologous endoglucanases, which have been proposed to play a role in secondary cell wall development, were examined. The class B endoglucanases, PtGH9B5 and AtGH9B5, are secreted enzymes that have a predicted glycosylphosphatidylinositol anchor, while the class C endoglucanases, PtGH9C2 and AtGH9C2, are also predicted to be secreted but instead contain a carbohydrate-binding module. The poplar endoglucanases were expressed in Arabidopsis using both a 35S promoter and the Arabidopsis secondary cell wall-specific CesA8 promoter. Additionally, Arabidopsis t-DNA insertion lines and an RNAi construct was created to downregulate AtGH9C2 in Arabidopsis. All of the plant lines were examined for changes in cell morphology and patterning, growth and development, cell wall crystallinity, microfibril angle, and proportion of cell wall carbohydrates. Misregulation of PtGH9B5/AtGH9B5 resulted in changes in xylose content, while misregulation of PtGH9C2/AtGH9C2 resulted in changes in crystallinity, which was inversely correlated with changes in plant height and rosette diameter...Continue Reading

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Citations

Mar 11, 2015·Journal of Integrative Plant Biology
Jan 24, 2018·Journal of Integrative Plant Biology·Miao LiZhibiao Ye
Mar 6, 2018·Annual Review of Plant Biology·Lilan HongAdrienne H K Roeder
Mar 21, 2020·Frontiers in Plant Science·Francesco Pancaldi, Luisa M Trindade
Nov 15, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Francesco SestiliDomenico Lafiandra
Mar 12, 2020·International Journal of Molecular Sciences·Deok Hyun SeoGeupil Jang
Sep 6, 2017·Frontiers in Plant Science·Luís F GoulaoSara Amâncio
Sep 16, 2021·The Plant Cell·Jennette M CodjoeElizabeth S Haswell
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