PMID: 15715941Feb 18, 2005Paper

Endo-1,4-beta-xylanase B from Aspergillus cf. niger BCC14405 isolated in Thailand: purification, characterization and gene isolation

Journal of Biochemistry and Molecular Biology
Asano KrisanaKirtikara Kanyawim

Abstract

During the screening of xylanolytic enzymes from locally isolated fungi, one strain BCC14405, exhibited high enzyme activity with thermostability. This fugal strain was identified as Aspergillus cf. niger based on its morphological characteristics and internal transcribed spacer (ITS) sequences. An enzyme with xylanolytic activity from BCC14405 was later purified and characterized. It was found to have a molecular mass of ca. 21 kDa, an optimal pH of 5.0, and an optimal temperature of 55 degrees C. When tested using xylan from birchwood, it showed K(m) and V(max) values of 8.9 mg/ml and 11,100 U/mg, respectively. The enzyme was inhibited by CuSO(4) EDTA, and by FeSO(4) The homology of the 20-residue N-terminal protein sequence showed that the enzyme was an endo-1,4-beta-xylanase. The full-length gene encoding endo-1,4-beta-xylanase from BCC14405 was obtained by PCR amplification of its cDNA. The gene contained an open reading frame of 678 bp, encoding a 225 amino acid protein, which was identical to the endo-1,4-a-xylanase B previously identified in A. niger.

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Citations

Nov 23, 2006·Applied Microbiology and Biotechnology·Gui Min ZhangXian En Zhang
Jul 2, 2009·Applied Microbiology and Biotechnology·Sibtain AhmedAmer Jamil
Sep 4, 2010·The Protein Journal·Jeyaraman VikramathithanKotteazeth Srikumar
Aug 15, 2009·Applied Biochemistry and Biotechnology·Adriana Knob, Eleonora Cano Carmona
Aug 18, 2012·Applied Biochemistry and Biotechnology·Dorra DrissSemia Ellouz Chaabouni
Oct 3, 2013·Protein Expression and Purification·Thi Tuyen DoVan Thuat Nguyen
Aug 17, 2010·FEMS Yeast Research·Niran RoongsawangSutipa Tanapongpipat
Jul 18, 2012·BMC Genomics·Mikael R AndersenJens Nielsen
Nov 9, 2005·Protein Expression and Purification·Duriya ChantasinghLily Eurwilaichitr
Oct 11, 2014·Bioprocess and Biosystems Engineering·Sheila Romo SánchezAna Briones Pérez
Dec 6, 2005·DNA Sequence : the Journal of DNA Sequencing and Mapping·Katewadee BoonyapakronLily Eurwilaichitr
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zahra AzzouzLaura Isabel de Eugenio
Jul 8, 2008·Protein Expression and Purification·Piyanun HarnpicharnchaiLily Eurwilaichitr

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