PMID: 1096Dec 18, 1975

Endo-arabinanase from Bacillus subtilis F-11

Biochimica Et Biophysica Acta
A Kaji, T Saheki

Abstract

An arabinanase was purified from the culture fluid of Bacillus subtilis F-11. The process was as follows: salting out by (NH4)2SO4, repeated chromatography on hydroxy apatite and gel filtration on Sepharose-6B. The purified enzyme was demonstrated to be homogeneous by disc electrophoresis. The enzyme was found to be active on arabinan and 1,5-arabinan, but inactive on phenyl alpha-L-arabinofuranoside, p-nitrophenyl beta-D-galactopyranoside, arabinoxylan, gum arabic. The enzyme released arabinose, arabinobiose, arabinotriose and higher oligosaccharides during the course of hydrolysis of 1,5-arabinan. The end products were found to be arabinose and arabinobiose after 144 h of hydrolysis.

Citations

Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A N Heyn
Jun 27, 2001·Applied and Environmental Microbiology·T Sakamoto, J F Thibault
Sep 11, 2007·Journal of Bacteriology·José Manuel Inácio, Isabel de Sá-Nogueira
Feb 20, 2004·Journal of Bacteriology·Maria Paiva RaposoIsabel de Sá-Nogueira
Jun 28, 2003·Carbohydrate Research·A K M Shofiqur RahmanKazuhiro Takamizawa
Nov 24, 2004·FEMS Microbiology Letters·Teresa Fontes Leal, Isabel de Sá-Nogueira
Oct 1, 1993·FEMS Microbiology Letters·D RamónJ Visser
Jan 1, 1997·Critical Reviews in Biotechnology·A Sunna, G Antranikian
Jan 1, 1989·Critical Reviews in Biotechnology·O P Ward, M Moo-Young
Oct 5, 2016·AMB Express·Seema A Belorkar, A K Gupta
Jan 1, 2019·Critical Reviews in Food Science and Nutrition·Wei XuWanmeng Mu

Related Concepts

Arabinose
Natto Bacteria
Molecular Sieve Chromatography
Glucosides
Exoglycosidases
Hydrogen-Ion Concentration
Mercury
Nitrophenylgalactosides
Oligosaccharides
Structure-Activity Relationship

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