Abstract
Endo-beta-mannosidase is a novel endoglycosidase that hydrolyzes the Manbeta1-4GlcNAc linkage in the trimannosyl core structure of N-glycans. This enzyme was partially purified and characterized in a previous report (Sasaki, A., Yamagishi, M., Mega, T., Norioka, S., Natsuka, S., and Hase, S. (1999) J. Biochem. 125, 363-367). Here we report the purification and molecular cloning of endo-beta-mannosidase. The enzyme purified from lily flowers gave a single band on native-PAGE and three bands on SDS-PAGE with molecular masses of 42, 31, and 28 kDa. Amino acid sequence information from these three polypeptides allowed the cloning of a homologous gene, AtEBM, from Arabidopsis thaliana. AtEBM was engineered for expression in Escherichia coli, and the recombinant protein comprised a single polypeptide chain with a molecular mass of 112 kDa corresponding to the sum of molecular masses of three polypeptides of the lily enzyme. The recombinant protein hydrolyzed pyridylamino derivatives (PA) of Manalpha1-6Manbeta1-4Glc-NAcbeta1-4GlcNAc into Manalpha1-6Man and GlcNAcbeta1-4Glc-NAc-PA, showing that AtEBM is an endo-beta-mannosidase. AtEBM hydrolyzed Man(n)Manalpha1-6Manbeta1-4GlcNAcbeta1-4GlcNAc-PA (n = 0-2) but not PA-sugar chains contain...Continue Reading
References
Jun 7, 1976·Biochimica Et Biophysica Acta·H Villarroya, F Petek
Dec 1, 1991·The Biochemical Journal·B Henrissat
Jul 1, 1990·Electrophoresis·H Hirano, T Watanabe
Mar 1, 1990·Analytical Biochemistry·H OkuT Ikenaka
Mar 22, 1985·Science·D J Lipman, W R Pearson
Aug 11, 1988·Nucleic Acids Research·R HiguchiR K Saiki
May 12, 1972·Biochimica Et Biophysica Acta·K SugaharaI Yamashina
Aug 15, 1970·Nature·U K Laemmli
Feb 24, 1995·The Journal of Biological Chemistry·H ChenK H Friderici
Nov 11, 1994·Nucleic Acids Research·J D ThompsonT J Gibson
Jun 1, 1996·The Biochemical Journal·B Henrissat, A Bairoch
May 1, 1996·Journal of Biochemistry·N HamagashiraS Hase
Oct 1, 1996·Genomics·J R LeipprandtM Z Jones
Dec 15, 1996·European Journal of Biochemistry·T IshimizuF Sakiyama
Mar 1, 1997·Glycobiology·P DurandJ P Mornon
Feb 28, 1998·Human Molecular Genetics·A H AlkhayatK H Friderici
Jan 9, 1998·Planta·J D BewleyG B Fincher
Apr 30, 1998·Journal of Chromatography. a·K YanagidaS Hase
Feb 17, 1999·Journal of Biochemistry·A SasakiS Hase
Mar 3, 1999·Bioscience, Biotechnology, and Biochemistry·G TakadaM Arai
May 29, 1999·Applied and Environmental Microbiology·D StollR A Warren
Sep 1, 1999·European Journal of Biochemistry·T IshimizuS Norioka
Apr 1, 2000·Applied and Environmental Microbiology·T ArakiT Morishita
May 19, 2001·European Journal of Biochemistry·P AdemarkJ Visser
Oct 9, 2001·Biotechnology and Bioengineering·K N ParkerR M Kelly
Mar 15, 2002·Bioscience Reports·T BeccariA Orlacchio
Apr 25, 2002·Glycobiology·Ranjani DhanawansaChristine H Scaman
Apr 5, 2003·Applied and Environmental Microbiology·Emese BékiJózsef Kukolya
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Citations
Nov 30, 2007·Planta·Zoran Minic
Aug 9, 2007·BMC Bioinformatics·Abdellali KelilAlain Fleury
Apr 9, 2014·PloS One·Taichi E TakasukaBrian G Fox
May 1, 2010·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Sumihiro Hase
Oct 24, 2013·Journal of Experimental Botany·Juan David RejónAntonio Jesús Castro
Jan 9, 2016·Critical Reviews in Biotechnology·Prakram Singh Chauhan, Naveen Gupta
Mar 30, 2005·The FEBS Journal·Akiko SasakiSumihiro Hase
Dec 8, 2017·The Biochemical Journal·Shun KatoTakeshi Ishimizu
Jun 22, 2006·Journal of Biochemistry·Takeshi IshimizuSumihiro Hase
Aug 30, 2012·Magnetic Resonance in Chemistry : MRC·Yumiko KubotaJun Uzawa
Sep 18, 2013·The Journal of Biological Chemistry·Simon LadevèzeGabrielle Potocki-Véronèse
Jul 28, 2019·International Journal of Biological Macromolecules·Kavyashree Sakharayapatna RanganathaSiva Kumar Nadimpalli
Feb 10, 2009·Phytochemistry·Renaud LéonardFriedrich Altmann