Endobronchial Ultrasound Reliably Quantifies Airway Smooth Muscle Remodeling in an Equine Asthma Model

PloS One
Michela BulloneJean-Pierre Lavoie

Abstract

Endobronchial ultrasonography (EBUS) revealed differences in the thickness of the layer representing subepithelial tissues (L2) between human asthmatics and controls, but whether this measurement correlates with airway smooth muscle (ASM) remodeling in asthma is unknown. In this study, we sought to determine the ability of EBUS to predict histological ASM remodeling in normal and equine asthmatic airways. We studied 109 isolated bronchi from the lungs of 13 horses. They underwent EBUS examination using a 30 MHz radial probe before being processed for histology. ASM remodeling parameters were evaluated in EBUS images (L2 thickness, L2 area, L2 area/internal perimeter [Pi] and L2 area/Pi2) and histological cuts (ASM area/Pi2), and compared. EBUS was then performed ex vivo on the lungs of 4 horses with heaves, an asthma-like condition of horses, and 7 controls to determine whether central bronchial remodeling could be detected with this technique. An optimized approach was developed based on data variability within airways, subjects, and groups, and then validated in 7 horses (3 controls, 4 with heaves) that underwent EBUS in vivo. L2 area was significantly associated to ASM area in isolated lungs (p<0.0001), in the absence of sig...Continue Reading

References

Jul 1, 1992·Thorax·T Hürter, P Hanrath
May 1, 1990·The American Review of Respiratory Disease·M EbinaM Tanemura
Jul 1, 1988·The American Review of Respiratory Disease·A L JamesP D Paré
Jun 1, 1993·Journal of Applied Physiology·R K LambertP D Paré
Apr 26, 2000·The European Respiratory Journal·A James, N Carroll
Dec 26, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Masayuki BabaTakehiko Fujisawa
Dec 10, 2002·American Journal of Respiratory and Critical Care Medicine·Alan L JamesNeil G Carroll
May 13, 2003·Chest·Armin ErnstUNKNOWN American College of Chest Physicians
May 13, 2003·Respiration; International Review of Thoracic Diseases·Franco FalconeDaniele Grosso
Jun 29, 2004·The European Respiratory Journal·T J ShawP H Howarth
Sep 15, 2005·The Journal of Allergy and Clinical Immunology·Carmela PepeQutayba Hamid
Aug 8, 2006·The Journal of Allergy and Clinical Immunology·Bérénice HerszbergJean-Pierre Lavoie
Apr 28, 2007·American Journal of Respiratory Cell and Molecular Biology·Madavi N OliverJeffrey J Fredberg
Sep 29, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y SumiQ Hamid
Mar 7, 2008·The European Respiratory Journal·B B AraujoT Mauad
Mar 14, 2009·The European Respiratory Journal·A L JamesF H Green
Jun 2, 2009·The Journal of Allergy and Clinical Immunology·Marta KaminskaJames Martin
Oct 12, 2010·American Journal of Respiratory Cell and Molecular Biology·Mathilde LeclereJean-Pierre Lavoie
Aug 10, 2011·Respirology : Official Journal of the Asian Pacific Society of Respirology·Mathilde LeclereJean-Pierre Lavoie
Oct 12, 2011·Journal of Veterinary Internal Medicine·K KoblingerR Léguillette
Mar 10, 2012·American Journal of Respiratory and Critical Care Medicine·Alan L JamesFrancis H Green
Oct 1, 2010·Journal of Bronchology & Interventional Pulmonology·Toshiyuki KitaShinji Nakao
Sep 7, 2013·Chest·Nobuaki Hirota, James G Martin
Jun 1, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Emilie L SetlakweJean-Pierre Lavoie
Sep 27, 2014·Respirology : Official Journal of the Asian Pacific Society of Respirology·John G ElliotAlan L James

❮ Previous
Next ❯

Citations

Jan 14, 2017·American Journal of Respiratory and Critical Care Medicine·Y S PrakashUNKNOWN ATS Assembly on Respiratory Structure and Function
Jan 10, 2017·The Journal of Allergy and Clinical Immunology·Abhaya TrivediMario Castro
Apr 8, 2017·Equine Veterinary Journal·M Bullone, J-P Lavoie
Feb 17, 2018·Journal of Leukocyte Biology·Helene F Rosenberg, Kirk M Druey
Nov 13, 2019·Cell and Tissue Research·Michela Bullone, Jean-Pierre Lavoie
Sep 10, 2020·Frontiers in Veterinary Science·Laurent CouetilCyprianna Swiderski
Aug 20, 2017·Scientific Reports·Michela BulloneJean-Pierre Lavoie
May 29, 2021·Journal of Veterinary Internal Medicine·Esther M Millares-Ramirez, Jean-Pierre Lavoie
Sep 2, 2021·Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere·Ann Kristin Barton, Heidrun Gehlen
Dec 9, 2021·Journal of Veterinary Internal Medicine·Amandine BessonnatJean-Pierre Lavoie

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
sedation
imaging technique
imaging
biopsy

Software Mentioned

ImageJ
SAS
Photoshop
iMovie
ARRIVE

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Related Papers

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
Ping-ping ChenXiu-yu Li
American Journal of Physiology. Lung Cellular and Molecular Physiology
Mylène ChevignyJean-Pierre Lavoie
The Journal of Allergy and Clinical Immunology
Bérénice HerszbergJean-Pierre Lavoie
© 2021 Meta ULC. All rights reserved