Endocytic function is critical for influenza A virus infection via DC-SIGN and L-SIGN

Scientific Reports
Leah GillespieSarah L Londrigan

Abstract

The ubiquitous presence of cell-surface sialic acid (SIA) has complicated efforts to identify specific transmembrane glycoproteins that function as bone fide entry receptors for influenza A virus (IAV) infection. The C-type lectin receptors (CLRs) DC-SIGN (CD209) and L-SIGN (CD209L) enhance IAV infection however it is not known if they act as attachment factors, passing virions to other unknown receptors for virus entry, or as authentic entry receptors for CLR-mediated virus uptake and infection. Sialic acid-deficient Lec2 Chinese Hamster Ovary (CHO) cell lines were resistant to IAV infection whereas expression of DC-SIGN/L-SIGN restored susceptibility of Lec2 cells to pH- and dynamin-dependent infection. Moreover, Lec2 cells expressing endocytosis-defective DC-SIGN/L-SIGN retained capacity to bind IAV but showed reduced susceptibility to infection. These studies confirm that DC-SIGN and L-SIGN are authentic endocytic receptors for IAV entry and infection.

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Citations

May 24, 2016·Viruses·Amelina AlbornozNicole D Tischler
Mar 23, 2017·Viruses·João T Monteiro, Bernd Lepenies
Jul 21, 2016·Current Environmental Health Reports·Christopher A LowryGraham A W Rook
Jan 29, 2020·Cellular Microbiology·Tina MeischelSarah L Londrigan
May 20, 2020·Chembiochem : a European Journal of Chemical Biology·Pablo ValverdeJesús Jiménez-Barbero
Jun 22, 2017·Bioscience, Biotechnology, and Biochemistry·Hirokazu YagiMasamichi Tsuboi
May 8, 2020·Viruses·Hi Eun Jung, Heung Kyu Lee
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Jul 20, 2019·Scientific Reports·Anna MoskovskichGiulio Superti-Furga
Sep 24, 2020·Vaccines·Hye Suk HwangYoong Ahm Kim
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Apr 13, 2021·Biomedical Research·Hideki YamamotoKazuyoshi Kawakami
Oct 9, 2021·Clinical Science·Laura MarongiuFrancesca Granucci

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Methods Mentioned

BETA
glycosylation
FCS
flow cytometry
confocal microscopy

Software Mentioned

Zen2012
GraphPad
Image J
GraphPad Prism

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