Endocytic proteins drive vesicle growth via instability in high membrane tension environment

Proceedings of the National Academy of Sciences of the United States of America
Nikhil WalaniAshutosh Agrawal

Abstract

Clathrin-mediated endocytosis (CME) is a key pathway for transporting cargo into cells via membrane vesicles; it plays an integral role in nutrient import, signal transduction, neurotransmission, and cellular entry of pathogens and drug-carrying nanoparticles. Because CME entails substantial local remodeling of the plasma membrane, the presence of membrane tension offers resistance to bending and hence, vesicle formation. Experiments show that in such high-tension conditions, actin dynamics is required to carry out CME successfully. In this study, we build on these pioneering experimental studies to provide fundamental mechanistic insights into the roles of two key endocytic proteins-namely, actin and BAR proteins-in driving vesicle formation in high membrane tension environment. Our study reveals an actin force-induced "snap-through instability" that triggers a rapid shape transition from a shallow invagination to a highly invaginated tubular structure. We show that the association of BAR proteins stabilizes vesicles and induces a milder instability. In addition, we present a rather counterintuitive role of BAR depolymerization in regulating the shape evolution of vesicles. We show that the dissociation of BAR proteins, suppor...Continue Reading

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Citations

Feb 22, 2016·Journal of the Mechanical Behavior of Biomedical Materials·L DeseriK Dayal
Sep 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Mehdi TorbatiAshutosh Agrawal
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