Endocytic Rabs Are Recruited to the Trypanosoma cruzi Parasitophorous Vacuole and Contribute to the Process of Infection in Non-professional Phagocytic Cells.

Frontiers in Cellular and Infection Microbiology
Betiana Nebaí SalassaPatricia Silvia Romano

Abstract

Trypanosoma cruzi is the parasite causative of Chagas disease, a highly disseminated illness endemic in Latin-American countries. T. cruzi has a complex life cycle that involves mammalian hosts and insect vectors both of which exhibits different parasitic forms. Trypomastigotes are the infective forms capable to invade several types of host cells from mammals. T. cruzi infection process comprises two sequential steps, the formation and the maturation of the Trypanosoma cruzi parasitophorous vacuole. Host Rab GTPases are proteins that control the intracellular vesicular traffic by regulating budding, transport, docking, and tethering of vesicles. From over 70 Rab GTPases identified in mammalian cells only two, Rab5 and Rab7 have been found in the T. cruzi vacuole to date. In this work, we have characterized the role of the endocytic, recycling, and secretory routes in the T. cruzi infection process in CHO cells, by studying the most representative Rabs of these pathways. We found that endocytic Rabs are selectively recruited to the vacuole of T. cruzi, among them Rab22a, Rab5, and Rab21 right away after the infection followed by Rab7 and Rab39a at later times. However, neither recycling nor secretory Rabs were present in the vac...Continue Reading

References

Mar 8, 1991·Cell·J P GorvelJ Gruenberg
Aug 1, 1993·Current Opinion in Cell Biology·M Zerial, H Stenmark
Jul 1, 1996·The Journal of Cell Biology·A RodríguezN W Andrews
Mar 2, 1999·Nature·S ChristoforidisM Zerial
Feb 26, 2000·Molecular Biology of the Cell·C BucciB van Deurs
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F SealsA Price
Jan 2, 2001·Biology of the Cell·S Martinez-ArcaT Galli
Feb 22, 2002·Biochemical and Biophysical Research Communications·S E WilkowskyE L D Isola
Apr 10, 2003·Biochemical and Biophysical Research Communications·Taoyong ChenXuetao Cao
Jul 24, 2003·Journal of Cell Science·Aaron M WoolseyBarbara A Burleigh
Jul 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Oliver M SchlüterThomas C Südhof
Nov 3, 2004·The Journal of Experimental Medicine·Luciana O Andrade, Norma W Andrews
Nov 25, 2004·Journal of Cell Science·Jeremy C SimpsonArwyn T Jones
Jul 13, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Denise G J BatistaMaria N C Soeiro
Sep 22, 2005·Nature Reviews. Microbiology·Luciana O Andrade, Norma W Andrews
Mar 16, 2006·Molecular and Cellular Biology·Javier G MagadánLuis S Mayorga
Jun 17, 2009·Cell Host & Microbe·Yadunanda Kumar, Raphael H Valdivia
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Félix E Rivera-Molina, Peter J Novick
Aug 22, 2009·Nature Reviews. Molecular Cell Biology·Barth D Grant, Julie G Donaldson
Sep 18, 2009·Molecular Biology of the Cell·Huaiping ZhuGuangpu Li
May 4, 2011·The Journal of Experimental Medicine·Maria Cecilia FernandesNorma W Andrews
Oct 25, 2014·Cold Spring Harbor Perspectives in Biology·Angela Wandinger-Ness, Marino Zerial
Nov 9, 2014·Journal of Cell Science·Maria Cecilia FernandesNorma W Andrews
Feb 11, 2015·Annual Review of Physiology·Haoxing Xu, Dejian Ren
Aug 15, 2015·Journal of Cell Science·Yan Zhen, Harald Stenmark
Jun 7, 2017·PLoS Neglected Tropical Diseases·Natália Fernanda CoutoLuciana O Andrade
Nov 2, 2017·PLoS Neglected Tropical Diseases·María Cristina VanrellPatricia Silvia Romano
Apr 17, 2019·International Journal of Molecular Sciences·Julián Gambarte TudelaMaría Teresa Damiani

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Citations

Mar 23, 2021·Frontiers in Cellular and Infection Microbiology·Gabriel Ferri, Martin M Edreira

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Methods Mentioned

BETA
GTPases
confocal microscopy

Software Mentioned

Addgene

Related Concepts