Endocytosis in cultured neurons is altered by chronic alcohol exposure

Toxicological Sciences : an Official Journal of the Society of Toxicology
María P MarínJaime Renau-Piqueras

Abstract

Endocytosis is required for many cellular pivotal processes, including membrane recycling, nutrient uptake, and signal transduction. This complex process is particularly relevant in polarized cells, such as neurons. Previous studies have demonstrated that alcohol alters intracellular traffic, including endocytosis, in several cell types. However, information on the effect of chronic alcohol exposure on this process in neurons is scarce. As an approach, we investigated the effect of alcohol exposure on the internalization of two widely used endocytic markers, albumin and transferrin, in developing hippocampal neurons in primary culture. The effect of this treatment on the levels of several representative proteins involved in the endocytic process was also analyzed. Some of these proteins are also involved in the organization of the actin cytoskeleton. Pretreatment of cells with inhibitors chlorpromazine or nystatin indicates that albumin is internalized mainly by caveolin-dependent endocytosis. On the other hand, alcohol decreases the endocytosis of both markers, although no qualitative changes in the distribution of either of these molecules were observed. Finally, the effect of ethanol on the proteins analyzed was heterogeneou...Continue Reading

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Citations

Jul 4, 2013·Neurotoxicity Research·Ana M RomeroGuillermo Esteban-Pretel
Oct 19, 2011·ACS Nano·Cameron W EvansK Swaminathan Iyer
Dec 3, 2010·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Raúl BallestínXavier Ponsoda
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Nov 3, 2012·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Guillermo Esteban-PretelJaime Renau-Piqueras
Jul 16, 2014·Neurotoxicity Research·Ana M RomeroGuillermo Esteban-Pretel
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Jun 11, 2011·Critical Reviews in Clinical Laboratory Sciences·Silvia Alfonso-Loeches, Consuelo Guerri
May 2, 2021·Molecular Neurobiology·Neide VieiraNuno Sousa

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