Endocytosis-mediated siderophore uptake as a strategy for Fe acquisition in diatoms

Science Advances
Elena KazamiaEmmanuel Lesuisse

Abstract

Phytoplankton growth is limited in vast oceanic regions by the low bioavailability of iron. Iron fertilization often results in diatom blooms, yet the physiological underpinnings for how diatoms survive in chronically iron-limited waters and outcompete other phytoplankton when iron becomes available are unresolved. We show that some diatoms can use siderophore-bound iron, and exhibit a species-specific recognition for siderophore types. In Phaeodactylum tricornutum, hydroxamate siderophores are taken up without previous reduction by a high-affinity mechanism that involves binding to the cell surface followed by endocytosis-mediated uptake and delivery to the chloroplast. The affinity recorded is the highest ever described for an iron transport system in any eukaryotic cell. Collectively, our observations suggest that there are likely a variety of iron uptake mechanisms in diatoms besides the well-established reductive mechanism. We show that iron starvation-induced protein 1 (ISIP1) plays an important role in the uptake of siderophores, and through bioinformatics analyses we deduce that this protein is largely diatom-specific. We quantify expression of ISIP1 in the global ocean by querying the Tara Oceans atlas of eukaryotic ge...Continue Reading

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Citations

Aug 18, 2018·Environmental Microbiology·Natalie R CohenAdrian Marchetti
Apr 9, 2019·Environmental Microbiology·Pavla DebeljakIngrid Obernosterer
Apr 14, 2020·Environmental Microbiology·Mercedes Nieves-MoriónRachel A Foster
Nov 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tyler H CoaleAndrew E Allen
Jan 10, 2021·Proceedings of the National Academy of Sciences of the United States of America·Richard G DorrellGuillaume Blanc
Dec 1, 2020·Frontiers in Microbiology·Robert SutakEmmanuel Lesuisse
Mar 12, 2021·Journal of Experimental Botany·Xia GaoElena Kazamia
Jul 22, 2021·Gels·Peter H SantschiRuei-Feng Shiu
Jul 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Loay J JabreErin M Bertrand
Jan 1, 2021·Environmental Science & Technology·Eshani HettiarachchiGayan Rubasinghege
Oct 13, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Matthias MisslingerHubertus Haas

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Methods Mentioned

BETA
transgenic
flow cytometry
dissection
PCR
electrophoresis

Software Mentioned

Quantity One
SWISS
PFAM
R
CIPRES
HMMER
MODEL
SignalP
MAFFT
MrBayes

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