PMID: 8601582Mar 1, 1996Paper

Endocytosis of activated receptors and clathrin-coated pit formation: deciphering the chicken or egg relationship

The Journal of Cell Biology
F Santini, J H Keen

Abstract

The fundamental mechanisms by which receptors once targeted for endocytosis are found in coated pits is an important yet unresolved question. Specifically, are activated receptors simply trapped on encountering preexisting coated pits, subsequently being rapidly internalized? Or do the receptors themselves, by active recruitment, gather soluble coat and cytosolic components and initiate the rapid assembly of new coated pits that then mediate their internalization? To explore this question, we studied the relationship between activation of IgE-bound high affinity Fc receptors (FCepsilonRI) and coated pit formation. Because these receptors are rapidly internalized via clathrin-coated pits only when cross-linked by the binding of multivalent antigens, we were able to separate activation from internalization by using an immobilized antigen. The FCepsilonRIs, initially uniformly distributed over the cell surface. relocalized and aggregated on the antigen-exposed membrane. The process was specific for the antigen, and temperature- and time-dependent. This stimulation initiated a cascade of cellular responses typical of FCepsilonRI signaling including membrane ruffling, cytoskeletal rearrangements, and, in the presence of Ca2+, exocyt...Continue Reading

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Citations

Jan 1, 1997·Annual Review of Biochemistry·S L Schmid
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A SubtilT E McGraw
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