Endocytosis of cationic and anionic proteins in cultivated arterial smooth muscle cells

Experimental Cell Research
J NilssonJ Thyberg

Abstract

Confluent secondary cultures of rat arterial smooth muscle cells were exposed to cationic and anionic derivatives of ferritin and horseradish peroxidase and studied electron microscopically in order to clarify the influence of molecular net charge on surface binding and endocytosis of proteins. The cationic markers bound uniformly to the plasma membrane. They were then ingested by membrane invagination and via small vesicles transported to lysosomes and the Golgi complex. These organelles were both labelled already after 30 min of incubation. With longer exposure times (2-4 h), an increasing accumulation within the lysosomes was observed, whereas the labelling of the Golgi complex decreased. In spite of continued interiorization of plasma membrane carrying the cationic markers, the cells retained their ability to bind the latter to the surface. The anionic markers did not bind to the cell surface, were taken up in the fluid phase, and later observed only in lysosomes. If assuming that the cationic and anionic proteins serve as markers for the plasma membrane and fluid phase, respectively, but do not affect the intracellular path of interiorized membrane, these results indicate that the endocytic vesicles fuse with and empty the...Continue Reading

References

Oct 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H G Rennke, M A Venkatachalam
Jan 1, 1977·Annual Review of Biochemistry·S C SilversteinZ A Cohn
Jun 1, 1978·Cell·R Ross, A Vogel
Oct 1, 1972·The Journal of Cell Biology·R M Steinman, Z A Cohn
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Jan 1, 1966·Annual Review of Physiology·C De Duve, R Wattiaux
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
Jul 1, 1980·The Journal of Experimental Medicine·P D OttosenM G Farquhar
Oct 1, 1981·Experimental Cell Research·E H NielsenP Bytzer
Jan 1, 1981·Annual Review of Physiology·I H Pastan, M C Willingham
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C H HeldinA Wasteson
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H N Antoniades

Citations

Jan 1, 1987·Acta histochemica·I L Leknes
Dec 1, 1988·The Journal of Cell Biology·S FroshauerA Helenius
Feb 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E M RosenD W Vinter
Feb 21, 1992·European Journal of Protistology·M Christofidou-Solomidou, W Stockem

Related Concepts

Polycationic ferritin
Metazoa
Anions
Ascending Aorta Structure
Cations
Plasma Membrane
Electrochemistry
Endocytosis
Isoferritin
Golgi Apparatus

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