Endocytosis of follicle-stimulating hormone by ovarian granulosa cells: analysis of hormone processing and receptor dynamics

Journal of Cellular Physiology
J C Sanford, B E Batten

Abstract

Suspensions of freshly isolated rat granulosa cells were used to study endocytosis and processing of radioiodinated ovine follicle-stimulating hormone (I-oFSH) and to analyze the dynamics of its receptor. Ovine FSH was iodinated to a specific activity of 26 microCi/micrograms as determined by radioreceptor self-displacement assays with maximum specific binding to excess membrane receptors of 46%. Radiolabeled oFSH was judged biologically equivalent to the unlabeled hormone since I-oFSH shows saturation-binding kinetics and stimulates steroidogenesis in a similar dose-related manner to unlabeled oFSH. Experiments designed to study the extent and time course of degradation involved continuous exposure of isolated granulosa cells to I-oFSH. Saturation of membrane receptors was achieved within 1.5 h of incubation, and internalization of FSH occurred in a linear manner for up to 6 h. The rate of internalization was equivalent to 2,780 FSH molecules/cell/h. Degradation of FSH became apparent after 6 h of incubation and increased to 86% of total cellular-associated radioactivity at 22 h. FSH degradation was inhibited by 100 microM chloroquine or 0.45 mM leupeptin. The measurement of cell surface I-oFSH binding in the combined presence...Continue Reading

References

Jan 1, 1976·Advances in Protein Chemistry·P Cuatrecasas, M D Hollenberg
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R M Smith, L Jarett
Aug 15, 1973·Nature: New Biology·K J Catt, M L Dufau
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jan 1, 1981·Annual Review of Physiology·I H Pastan, M C Willingham
Jul 9, 1981·The New England Journal of Medicine·A C King, P Cuatrecasas
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D I Buckley, J Ramachandran
Aug 1, 1982·Endocrinology·M M Sanders, A R Midgley
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·M S BrownJ L Goldstein
Jul 1, 1982·The Journal of Cell Biology·M C Willingham, I H Pastan
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F PurrelloI D Goldfine
Oct 1, 1983·Journal of Cellular Physiology·M S RobinsonD F Albertini
Jan 1, 1984·Molecular and Cellular Endocrinology·P W Fletcher, L E Reichert
Jan 15, 1983·The Biochemical Journal·J M Besterman, R B Low
Jun 1, 1983·The Journal of Cell Biology·J M BestermanD E Rannels
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

❮ Previous
Next ❯

Citations

May 30, 2012·Molecular and Cellular Endocrinology·Madhavi DupakuntlaSmita D Mahale
Oct 23, 2010·Theriogenology·E J C VerbraakC Zijlstra
Oct 15, 2009·Molecular and Cellular Endocrinology·Madhavi Dupakuntla, Smita D Mahale
Feb 7, 2007·Journal of Reproductive Immunology·J D GhosalkarS D Mahale
Aug 27, 2010·Biology of Reproduction·Jitu W GeorgeLeslie L Heckert
Jun 12, 2019·Clinical and Experimental Reproductive Medicine·Sirin Bakti DemirayUgur Sezerman
Oct 17, 2020·Biochimica Et Biophysica Acta. Biomembranes·Kaushiki S PrabhudesaiSusan Idicula-Thomas
May 23, 2021·Bioorganic & Medicinal Chemistry Letters·Kaushiki S PrabhudesaiSusan Idicula-Thomas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.