Endocytosis of GABAB receptors modulates membrane excitability in the single-celled organism Paramecium

Journal of Cell Science
Paola RamoinoCesare Usai

Abstract

GABAB receptors modulate swimming behavior in Paramecium by inhibiting dihydropyridine-sensitive Ca2+ channels via G-proteins. Prolonged occupancy of GABAB receptors by baclofen results in a decrease in GABAB receptor functions. Since changes in the number of cell-surface GABAA receptors have been postulated to be of importance in modulating inhibitory synaptic transmission in neurons, we have studied the cell-surface expression and maintenance of GABAB receptors in P. primaurelia. In this study, we use immunostaining in electron and confocal microscopy to demonstrate that constitutive internalization of GABAB receptors in P. primaurelia is mediated by clathrin-dependent and -independent endocytosis. Indeed, GABAB receptors colocalize with the adaptin complex AP2, which is implicated in the selective recruitment of integral membrane proteins to clathrin-coated vesicles, and with caveolin 1, which is associated with uncoated membrane invaginations. Furthermore, when endocytosis is blocked with hypertonic medium, cytosol acidification, filipin or with a peptide that disrupts the association between amphiphysin and dynamin, the effect of baclofen on swimming is increased. These results suggest that GABAB receptor endocytosis into ...Continue Reading

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Citations

Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Miho TerunumaAndrés Couve
Sep 27, 2008·Pakistan Journal of Biological Sciences : PJBS·M Saeed Heydarnejad
Jul 31, 2007·Microscopy Research and Technique·Paola RamoinoRenata Manconi
Mar 4, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Paola RamoinoRenata Manconi
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Dec 23, 2016·Frontiers in Neuroanatomy·Daniel Romaus-SanjurjoMaría Celina Rodicio
Oct 22, 2013·The Journal of Experimental Biology·Paola RamoinoGiambattista Bonanno

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