PMID: 7537102Apr 13, 1995Paper

Endocytosis of poly(ethylene oxide)-modified liposome by human lymphoblastoid cells

Biochimica Et Biophysica Acta
N Higashi, J Sunamoto

Abstract

Egg PC liposome as reconstituted with poly(ethylene oxide)-bearing lipid (coded as PEO-lipid (n = 15)) was remarkably endocytosed by Jurkat cell, which was a lymphoblastoma derived from human T cell. To confirm the endocytosis, two kinds of fluorescent probes (FITC-dextran and octadecyl rhodamine B) were employed. The former was loaded in the aqueous phase of the liposome, while the latter was embedded in the liposomal membrane. Both probes were found coincidentally at the same site in the cytosol, clearly suggesting that whole liposome entered the cell. The endocytosis was most obvious when PEO-lipid (n = 15) was employed above 50 mol%. FITC-Dextran entered the cell was found small dots in the cell, not dispersive. Even when octadecyl rhodamine B was used, no membrane fluorescence was observed at all. The uptake closely related to the cell metabolism as affected by the culture temperature and serum in the incubation medium. Furthermore, the addition of cytochalasin B completely prohibited the cell uptake of liposomes.

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Citations

Mar 4, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·D LetourneurM Cansell
Jul 14, 2009·Colloids and Surfaces. B, Biointerfaces·Kien Xuan NgoRyoichi Kuboi
Dec 4, 1996·Biochimica Et Biophysica Acta·N HigashiJ Sunamoto
Jun 22, 2000·Molecular Reproduction and Development·R CarballadaP Esponda
Jul 9, 1999·Journal of Biomedical Materials Research·M CansellD Letourneur
Nov 18, 2005·Journal of Colloid and Interface Science·Alexandros PantosConstantinos M Paleos
Jul 14, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Alexandros PantosGeorge Nounesis
Jul 13, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Alexandros PantosGeorge Nounesis

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