Endodermal differentiation of murine embryonic carcinoma cells by retinoic acid requires JLP, a JNK-scaffolding protein

Journal of Cellular Biochemistry
K KashefDanny N Dhanasekaran

Abstract

Retinoic acid (RA) is a morphogen that induces endodermal differentiation of murine P19 embryonic carcinoma cells. RA-induced differentiation of P19 cells has been used as a model system to define the differentiation programs of pluripotent stem cells. Using this system it has been shown that G alpha13--the alpha-subunit of the heterotrimeric G protein G13--and its activation of JNK-module are critically required for the endodermal differentiation of P19 cells. However, the mechanism through which G alpha13 is linked to JNK-module is unknown. Here, we report that RA stimulates the expression of JNK-interacting leucine zipper protein (JLP), a newly identified JNK-scaffolding protein and its critical role in RA-mediated endodermal differentiation. Our results indicate that there is a physical association between JLP and G alpha13 in RA-stimulated P19 cells. More interestingly, silencing JLP abrogates RA-mediated endodermal differentiation of P19 cells analogous to the effects seen with the silencing of G alpha13 or JNK. Therefore, our studies presented here identify for the first time, a novel role for a newly identified scaffolding protein in RA-mediated endodermal differentiation, providing a new signaling conduit to transmit s...Continue Reading

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Citations

Aug 13, 2011·Molecular and Cellular Biochemistry·Jyotshnabala Kanungo, Settara C Chandrasekharappa
May 15, 2007·Oncogene·Z G Goldsmith, D N Dhanasekaran
May 15, 2007·Oncogene·D N DhanasekaranE P Reddy
Dec 6, 2008·The Journal of Biological Chemistry·Ognian C IkonomovAssia Shisheva
Nov 1, 2007·Journal of Molecular Signaling·Alexandra V AndreevaTatyana A Voyno-Yasenetskaya
May 9, 2009·British Journal of Pharmacology·Juhi Juneja, Patrick J Casey
Nov 20, 2009·Cell Proliferation·W EngströmK Moorwood
Jan 15, 2015·The Journal of Biological Chemistry·Hui-ming WangGuo-hua Ding
Feb 5, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Deepika KanojiaAnil Suri

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