Endogenous alphaCGRP protects against concanavalin A-induced hepatitis in mice

Biochemical and Biophysical Research Communications
Akiko KamiyoshiTakayuki Shindo

Abstract

To evaluate hepatoprotective effect of alpha-calcitonin gene-related peptide (alphaCGRP), we compared the susceptibilities of alphaCGRP-/- and wild-type mice to concanavalin A (Con A)-induced hepatitis. Twelve hours after Con A administration, serum transaminases were markedly higher in alphaCGRP-/- than wild-type mice, and much more extensive TUNEL-positive lesions and DNA fragmentation were detected in the livers of alphaCGRP-/- mice. Notably, expression of IL-6 was selectively diminished in alphaCGRP-/- mice, suggesting that induction of IL-6 during acute inflammatory responses is blunted in alphaCGRP-/- mice. In addition, primary cultured alphaCGRP-/- hepatocytes were more susceptible to IFN-gamma-induced cell death than hepatocytes from wild-type mice. Administration of exogenous alphaCGRP reduced the incidence of apoptosis among hepatocytes and endothelial cells. It thus appears that alphaCGRP exerts a hepatoprotective effect by modulating cytokine expression and preventing apoptosis.

References

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Jun 29, 2001·Journal of Gastroenterology·M YonedaA Terano

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Citations

Jan 23, 2016·Progress in Retinal and Eye Research·Yasuhiro IesatoYasuo Yanagi
Aug 5, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Akiko KamiyoshiTakayuki Shindo
Aug 30, 2008·Protein Science : a Publication of the Protein Society·Seisuke KusanoShigeyuki Yokoyama
Jan 29, 2016·Scientific Reports·Takayuki SakuraiTakayuki Shindo
Jan 4, 2015·The Journal of Surgical Research·Apostolos N KandilisDina G Tiniakos
Nov 22, 2018·PloS One·Tomoyoshi InoueMasataka Majima

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