Endogenous and exogenous galectin-3 promote the adhesion of tumor cells with low expression of MUC1 to HUVECs through upregulation of N-cadherin and CD44

Laboratory Investigation; a Journal of Technical Methods and Pathology
Zhanqi CaoXiuli Guo

Abstract

Tumor cell-endothelial adhesion is one of the key steps in tumor cell haematogenous dissemination in metastasis and was previously shown to be mediated by interaction of galectin-3 with the transmembrane mucin protein MUC1. In this study, the effect of exogenous as well as endogenous galectin-3 on adhesion of two cell lines (low MUC1-expressing human prostate cancer PC-3M cells and non-small-cell lung cancer A549 cells) to monolayer of umbilical vein endothelial cells (HUVECs) was investigated. We found that suppression of endogenous galectin-3 expression reduced tumor cell adhesion to HUVECs and also decreased cell invasion and migration. Exogenous galectin-3 promoted tumor cell adhesion to HUVECs by entering cells. Both exogenous and endogenous galectin-3 upregulated the expression of β-catenin and increased β-catenin nuclear accumulation, and subsequently upregulated the expression of N-cadherin and CD44. We deduced that both exogenous as well as endogenous galectin-3 promoted low MUC1-expressing cancer cell adhesion to HUVECs by increasing the expression of N-cadherin and CD44 via an increase of nuclear β-catenin accumulation. These results were confirmed further by using a β-catenin/TCF transcriptional activity inhibitor, ...Continue Reading

References

Sep 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C KainzG Breitenecker
Feb 19, 1998·Biochimica Et Biophysica Acta·J OchiengP Warfield
Jan 20, 1999·Molecular Pathology : MP·R J Sneath, D C Mangham
Feb 23, 1999·The American Journal of Pathology·V J WielengaS T Pals
Jul 20, 1999·Experimental and Molecular Pathology·A Herrera-Gayol, S Jothy
Nov 5, 1999·Advances in Cancer Research·V J WielengaS T Pals
Mar 1, 2000·Molecular Pathology : MP·S GoodisonD Tarin
Mar 7, 2000·The American Journal of Pathology·P Nangia-MakkerA Raz
Jul 4, 2002·Cell Biology International·Kwonseop KimElizabeth D Hay
Mar 6, 2004·Cancer Metastasis Reviews·Stanley Zucker, Jeffrey Vacirca
Jun 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sanae NakajimaMasayuki Imamura
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Katayoon H EmamiMichael Kahn
Sep 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Keiji YoshinagaMasaki Mori
Sep 18, 2004·Cancer Research·Tatsuo ShimuraAvraham Raz
Jan 5, 2005·Nature Reviews. Cancer·Fu-Tong Liu, Gabriel A Rabinovich
Feb 16, 2006·Biochimica Et Biophysica Acta·Jerka DumicMirna Flögel
Apr 4, 2006·Molecular and Cellular Biology·Annick LaganaIvan R Nabi
May 22, 2007·Biochemical and Biophysical Research Communications·Zhou YangXiaoting Wu
Mar 20, 2008·Nature Clinical Practice. Oncology·Eva A TurleyMina J Bissell
Sep 23, 2008·The Journal of Biological Chemistry·Jens FriedrichsJonne Helenius
Nov 6, 2009·Molecular Cancer Therapeutics·Maya Datt JoshiDonald Kufe
Mar 18, 2010·Endocrine-related Cancer·Karin JennbackenKarin Welén
Jun 10, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fumi Takahashi-Yanaga, Michael Kahn
Dec 17, 2010·World Journal of Gastrointestinal Oncology·Lu-Gang Yu
Aug 21, 2014·Chembiochem : a European Journal of Chemical Biology·Anna K MichelMary J Cloninger

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation assay
transfection
electrophoresis
light microscopy
nuclear translocation

Software Mentioned

SPSS
Win

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.