PMID: 7541561Jan 1, 1995Paper

Endogenous and transfected mouse alpha-fetoprotein genes in undifferentiated F9 cells are activated in transient heterokaryons

Somatic Cell and Molecular Genetics
B T Spear, A W Ellis

Abstract

Mouse F9 teratocarcinoma cells provide a system to study developmentally regulated alpha-fetoprotein (AFP) gene expression. AFP is not expressed in undifferentiated F9 cells but is induced when cells differentiate as cell aggregates in the presence of retinoic acid. Previous studies have led to the suggestion that undifferentiated F9 cells contain negative regulators of AFP expression. To test this, we have used transient heterokaryons to ask whether inactive AFP genes in undifferentiated F9 cells are responsive to positively acting trans-acting factors. Our results indicate that silent endogenous and transfected AFP genes are activated when undifferentiated F9 cells are fused to human hepatoma HepG2 cells. This suggests that the lack of AFP expression in undifferentiated F9 cells is due to the absence or insufficient level of positive-acting transcription factors, rather than the presence of dominant negative regulators. We also demonstrate that stably transfected AFP genes, although unmethylated, are properly regulated in F9 cells.

References

Apr 1, 1990·The Journal of Cell Biology·A L TynerS M Tilghman
Jul 1, 1985·Molecular and Cellular Biology·R KrumlaufR L Brinster
Nov 15, 1985·Science·H M BlauC Webster
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·R KrumlaufS M Tilghman
Jan 25, 1988·Nucleic Acids Research·T F VogtS M Tilghman
Oct 1, 1989·Molecular and Cellular Biology·M H FeuermanS M Tilghman
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Dec 4, 1984·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S WrightF Grosveld
Jul 1, 1983·Molecular and Cellular Biology·R W Scott, S M Tilghman
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C L StewartR Jaenisch
Jun 11, 1994·Nucleic Acids Research·A JacobR R Reichel
Dec 1, 1993·Current Opinion in Cell Biology·M H Baron

❮ Previous
Next ❯

Citations

Nov 18, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anxun WangLingtao Wu
Oct 16, 2004·Molecular and Cellular Biology·Lingyun Long, Brett T Spear
Aug 3, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lorri A MorfordBrett T Spear
Aug 12, 1999·Seminars in Cell & Developmental Biology·R H Broyles
May 1, 1997·Experimental Cell Research·D J Denkinger, R S Kawahara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.