Endogenous apoC-I increases hyperlipidemia in apoE-knockout mice by stimulating VLDL production and inhibiting LPL.

Journal of Lipid Research
Marit WesterterpPatrick C N Rensen

Abstract

Previous studies have shown that overexpression of human apolipoprotein C-I (apoC-I) results in moderate hypercholesterolemia and severe hypertriglyceridemia in mice in the presence and absence of apoE. We assessed whether physiological endogenous apoC-I levels are sufficient to modulate plasma lipid levels independently of effects of apoE on lipid metabolism by comparing apolipoprotein E gene-deficient/apolipoprotein C-I gene-deficient (apoe-/-apoc1-/-), apoe-/-apoc1+/-, and apoe-/-apoc1+/+ mice. The presence of the apoC-I gene-dose-dependently increased plasma cholesterol (+45%; P < 0.001) and triglycerides (TGs) (+137%; P < 0.001), both specific for VLDL. Whereas apoC-I did not affect intestinal [3H]TG absorption, it increased the production rate of hepatic VLDL-TG (+35%; P < 0.05) and VLDL-[35S]apoB (+39%; P < 0.01). In addition, apoC-I increased the postprandial TG response to an intragastric olive oil load (+120%; P < 0.05) and decreased the uptake of [3H]TG-derived FFAs from intravenously administered VLDL-like emulsion particles by gonadal and perirenal white adipose tissue (WAT) (-34% and -25%, respectively; P < 0.05). As LPL is the main enzyme involved in the clearance of TG-derived FFAs by WAT, and total postheparin ...Continue Reading

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Citations

Aug 14, 2012·Journal of Neuroinflammation·Eiron CudabackChristopher Dirk Keene
Mar 6, 2009·Journal of Atherosclerosis and Thrombosis·Itsuko SatoShunichi Kumagai
Jun 29, 2010·Metabolism: Clinical and Experimental·Bob OlssonLena M S Carlsson
Mar 5, 2014·Proteomics. Clinical Applications·Joseph V MoxonJonathan Golledge
Nov 11, 2008·Biochemical and Biophysical Research Communications·Willeke de HaanPatrick C N Rensen
Apr 12, 2014·Biochimica Et Biophysica Acta·Sander Kersten
Jan 23, 2010·Molecular BioSystems·Diego DiezCraig E Wheelock
Dec 16, 2016·The Journal of Pathology·Pascal BusHans J Baelde
Mar 7, 2007·Journal of Lipid Research·Marit WesterterpPatrick C N Rensen
Sep 22, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Hanny WassefMay Faraj
Jan 19, 2018·PloS One·Jyotsna SridharanDaniel C Weinstein
Nov 30, 2019·International Journal of Molecular Sciences·Elena V Fuior, Anca V Gafencu
Dec 6, 2020·Trends in Endocrinology and Metabolism : TEM·Shuangcheng Alivia WuLing Qi
May 23, 2021·Molecular Biology Reports·Aybike Sena OzuynukNeslihan Coban

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