Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch.

The Journal of Biological Chemistry
Robyn M Murphy, Graham D Lamb

Abstract

Proteolytically active calpain-3/p94 is clearly vital for normal muscle function, since its absence leads to limb girdle muscular dystrophy 2A, but its function and regulatory control are poorly understood. Here we use single muscle fibers, individually skinned by microdissection, to investigate the diffusibility and autolytic activation of calpain-3 in situ. Virtually all calpain-3 present in mature muscle fibers is tightly bound in the vicinity of the titin N2A line and triad junctions and remains so irrespective of fiber stretching or raised [Ca(2+)]. Most calpain-3 is evidently bound within the contractile filament lattice, because (i) its slow diffusional loss is slowed further by locking myosin and actin into rigor and (ii) detergent dispersion of membranes causes rapid washout of most ryanodine receptors and sarcoplasmic reticulum Ca(2+) pumps with little accompanying washout of calpain-3. Calpain-3 autolyzes (becoming proteolytically active) in a tightly calcium-dependent manner. It remains in its nonactivated full-length form if [Ca(2+)] is maintained at < or = 50 nm, the normal resting level, even with brief increases to 2-20 mum during repeated tetanic contractions, but it becomes active (though still bound) if [Ca(2...Continue Reading

References

Jan 1, 1983·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·R B ArmstrongJ A Schwane
Jun 1, 1993·Pflügers Archiv : European journal of physiology·G M Stephenson, D G Stephenson
Mar 10, 1997·Journal of the Neurological Sciences·M J SpencerM Zatz
Jul 9, 1998·Journal of Applied Physiology·C P IngallsR B Armstrong
Aug 4, 2001·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·S BaghdiguianG Lefranc
Mar 15, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gordon L WarrenR B Armstrong
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·M J SpencerJ S Beckmann
Jun 26, 2003·The Journal of Physiology·Pasi TaviHåkan Westerblad
Jul 5, 2003·Physiological Reviews·Darrell E GollJinyang Cong
Oct 28, 2003·The American Journal of Pathology·Marina FaninCorrado Angelini
Nov 5, 2003·The Journal of Biological Chemistry·Yasuko OnoHiroyuki Sorimachi
Dec 4, 2003·Molecular and Cellular Biology·Mathieu TaveauIsabelle Richard
Apr 10, 2004·The Journal of Biological Chemistry·Beatriz Garcia DiazPeter L Davies
May 13, 2004·Human Molecular Genetics·I KramerovaMelissa J Spencer
Aug 10, 2004·Clinical and Experimental Pharmacology & Physiology·David L Morgan, Uwe Proske
Jan 22, 2005·The Biochemical Journal·Ravikiran RavulapalliPeter L Davies
Aug 19, 2005·American Journal of Physiology. Cell Physiology·Robyn M MurphyGraham D Lamb
Aug 24, 2005·Human Molecular Genetics·Kimberly A HuebschGregory A Cox
Feb 3, 2006·Journal of Muscle Research and Cell Motility·Koichi OjimaHiroyuki Sorimachi
Mar 15, 2006·Biochemistry·Beatriz E García DíazPeter L Davies
Jul 22, 2006·The Journal of Physiology·Robyn M MurphyGraham D Lamb
Aug 4, 2006·The FEBS Journal·Stéphanie DuguezIsabelle Richard
Mar 21, 2007·The Journal of Biological Chemistry·Koichi OjimaHiroyuki Sorimachi
Nov 1, 2008·Neuromuscular Disorders : NMD·Jacques S Beckmann, Melissa Spencer

❮ Previous
Next ❯

Citations

Mar 17, 2010·British Journal of Anaesthesia·P M Hopkins
Oct 2, 2009·Clinical and Experimental Pharmacology & Physiology·Robyn M Murphy
Mar 27, 2012·BMC Musculoskeletal Disorders·Simon HauerslevThomas O Krag
Aug 10, 2010·PloS One·Antoine de MorréeSilvère M van der Maarel
Feb 22, 2014·Journal of Muscle Research and Cell Motility·Keita KanzakiMasanobu Wada
Oct 9, 2014·Expert Reviews in Molecular Medicine·Ainara Vallejo-IllarramendiAdolfo López de Munain
Dec 17, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Beau D ReillyCraig E Franklin
Sep 13, 2015·Biochimie·Yasuko OnoHiroyuki Sorimachi
Jan 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ke LiuAllen Taylor
Dec 23, 2009·Journal of Molecular and Cellular Cardiology·Bryan D HudsonHenk L Granzier
Apr 17, 2015·Human Molecular Genetics·Karine ChartonBjarne Udd
Oct 30, 2014·Frontiers in Physiology·Aziz GuellichCatherine Coirault
Jun 16, 2017·American Journal of Physiology. Cell Physiology·Stefan G WetteRobyn M Murphy
Sep 27, 2018·The Journal of Biological Chemistry·Christian-Scott E McCartneyPeter L Davies
Mar 20, 2009·American Journal of Physiology. Cell Physiology·Esther VerburgGraham D Lamb
Jan 25, 2014·Journal of Applied Physiology·F MacalusoK H Myburgh
Oct 7, 2011·American Journal of Physiology. Cell Physiology·Noni T LarkinsGraham D Lamb
May 28, 2009·Journal of Applied Physiology·Robyn M Murphy, Graham D Lamb
Nov 17, 2010·The Journal of Physiology·Joseph Bruton
Jul 16, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Takashi SonobeYutaka Kano
Oct 10, 2014·American Journal of Physiology. Cell Physiology·Davi A G MázalaEva R Chin
Sep 28, 2012·American Journal of Physiology. Cell Physiology·Robyn M MurphyDavid I Stapleton
Apr 22, 2016·American Journal of Physiology. Cell Physiology·Hongyang XuRobyn M Murphy

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.