Endogenous factors derived from embryonic cortex regulate proliferation and neuronal differentiation of postnatal subventricular zone cell cultures

The European Journal of Neuroscience
Fabienne AgasseValérie Coronas

Abstract

In rodents, the subventricular zone (SVZ) harbours neural stem cells that proliferate and produce neurons throughout life. Previous studies showed that factors released by the developing cortex promote neurogenesis in the embryonic ventricular zone. In the present report, we studied in the rat the possible involvement of endogenous factors derived from the embryonic cortex in the regulation of the development of postnatal SVZ cells. To this end, SVZ neurospheres were maintained with explants or conditioned media (CM) prepared from embryonic day (E) 13, E16 or early postnatal cortex. We demonstrate that early postnatal cortex-derived factors have no significant effect on SVZ cell proliferation or differentiation. In contrast, E13 and E16 cortex release diffusible, heat-labile factors that promote SVZ cell expansion through increased proliferation and reduced cell death. In addition, E16 cortex-derived factors stimulate neuronal differentiation in both early postnatal and adult SVZ cultures. Fibroblast growth factor (FGF)-2- but not epidermal growth factor (EGF)-immunodepletion drastically reduces the mitogenic effect of E16 cortex CM, hence suggesting a major role of endogenous FGF-2 released by E16 cortex in the stimulation of ...Continue Reading

References

Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C Lois, A Alvarez-Buylla
Aug 26, 1998·Journal of Neurobiology·H A CameronR D McKay
May 18, 2000·Nature Neuroscience·A Björklund, O Lindvall
Aug 12, 2000·Neuroscience·M P NewmanA Mackay-Sim
Jul 16, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Frank-Peter WachsLudwig Aigner
Feb 20, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander Y MaslovSteven C Pruitt
Apr 28, 2004·Cytokine & Growth Factor Reviews·Richard Wing Chuen Wong, Laurent Guillaud
May 26, 2004·Transplant Immunology·Mark SaylesRoger A Barker

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