Endogenous fibrinolysis facilitates clot retraction in vivo.

Blood
Andre L SamsonS P Jackson

Abstract

Clot retraction refers to the process whereby activated platelets transduce contractile forces onto the fibrin network of a thrombus, which over time increases clot density and decreases clot size. This process is considered important for promoting clot stability and maintaining blood vessel patency. Insights into the mechanisms regulating clot retraction at sites of vascular injury have been hampered by a paucity of in vivo experimental models. By pairing localized vascular injury with thrombin microinjection in the mesenteric circulation of mice, we have demonstrated that the fibrin network of thrombi progressively compacts over a 2-hour period. This was a genuine retraction process, as treating thrombi with blebbistatin to inhibit myosin IIa-mediated platelet contractility prevented shrinkage of the fibrin network. Real-time confocal analysis of fibrinolysis after recombinant tissue-type plasminogen activator (tPA) administration revealed that incomplete proteolysis of fibrin polymers markedly facilitated clot retraction. Similarly, inhibiting endogenous fibrinolysis with tranexamic acid reduced retraction of fibrin polymers in vivo. In vitro clot retraction experiments indicated that subthreshold doses of tPA facilitated cl...Continue Reading

References

Nov 1, 1996·Journal of Magnetic Resonance Imaging : JMRI·K H TaberJ B Kirkpatrick
Apr 11, 2001·Molecular Biology of the Cell·J S BergR E Cheney
May 13, 2008·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Be'eri NiegoRobert L Medcalf
Jun 7, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Jocelyn M Auger, Steve P Watson
Aug 12, 2014·Stroke; a Journal of Cerebral Circulation·Marian MuchadaMarta Rubiera
Feb 12, 2015·Clinical Pharmacology and Therapeutics·P GurmanN M Elman
Apr 15, 2015·JAMA : the Journal of the American Medical Association·Shyam PrabhakaranRichard A Bernstein
May 15, 2015·Stroke; a Journal of Cerebral Circulation·Young Dae KimJi Hoe Heo

❮ Previous
Next ❯

Citations

May 21, 2018·Journal of Thrombosis and Haemostasis : JTH·T UranoY Suzuki
Dec 26, 2018·Journal of Thrombosis and Haemostasis : JTH·Valerie TutwilerJohn W Weisel
Jun 5, 2019·Expert Review of Hematology·Anetta Undas, Joanna Natorska
Feb 16, 2019·Anesthesia and Analgesia·Kenichi A TanakaEzeldeen Abuelkasem
Feb 16, 2019·Anesthesia and Analgesia·J Carolina Gomez-Builes, Sandro B Rizoli
Jun 13, 2020·Journal of Thrombosis and Thrombolysis·Diana A Gorog, Richard C Becker
Feb 26, 2019·The Journal of International Medical Research·Jun LuDeqin Geng
Jan 29, 2020·Research and Practice in Thrombosis and Haemostasis·Zhaoming TangAlisa S Wolberg
Feb 28, 2019·Blood Advances·Andreas MargrafMarkus Sperandio
Nov 25, 2020·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Jieliang ShenWei Jiang
Nov 15, 2020·Nature Communications·Leo NicolaiFlorian Gaertner
Mar 7, 2021·Biomolecules·Viki Raz LepskyAyelet Lesman
Mar 7, 2021·International Journal of Molecular Sciences·Claire S WhyteNicola J Mutch
Apr 21, 2021·Seminars in Thrombosis and Hemostasis·Maria J ColominaAntoni Sabate
Apr 21, 2021·Seminars in Thrombosis and Hemostasis·Anetta Undas
Jun 3, 2021·International Journal of Molecular Sciences·Rahim KanjiDiana A Gorog
Jul 18, 2021·Journal of Neurointerventional Surgery·Lukas MeyerUNKNOWN TOPMOST Study Group
Oct 6, 2021·International Journal of Laboratory Hematology·Pooja YadavSunil K Singh
Sep 28, 2021·Blood Advances·Karen P FongJoel S Bennett

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.