Abstract
In an accompanying study, we described the presence of intact fibronectin, a large adhesive molecule, in the specific granules of blood PMNs. Secretion of fibronectin by blood PMNs is poorly understood, and the fate of this fibronectin is practically unknown. In the present study we demonstrate that nanomolar concentrations of phorbol ester or the chemoattractants fMLP, PAF, and LTB4 induce fibronectin secretion from blood PMNs. Phorbol ester induced secretion of approximately 85% of the total fibronectin content, as well as expression of small amounts on the cell surface of the activated PMNs. Secreted fibronectin was proteolytically cleaved and, after 20 min, four major fragments of 150, 120, 90, and 80 kDa containing a midchain epitope were identified by Western blot analysis. Kinetic studies indicated that fibronectin was rapidly secreted as an intact molecule and that proteolysis started within minutes and proceeded for at least 1 h. If cells were removed after 5 min TPA treatment, no further proteolysis of the secreted fibronectin was observed, indicating participation of cell-bound proteinases. From a cocktail of proteinase inhibitors, PMSF was the most active in suppressing fibronectin proteolysis. Studies with specific...Continue Reading
References
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Apr 1, 1992·Journal of Leukocyte Biology·C A OwenR A Stockley
Jun 1, 1992·Journal of Leukocyte Biology·E Lundgren-AkerlundK E Arfors
Nov 1, 1992·Clinical and Experimental Immunology·H L Thompson, K Matsushima
Apr 3, 1992·Cell·R O Hynes
Oct 1, 1991·Journal of Leukocyte Biology·I DaudiP Vincent
Apr 1, 1990·Immunological Reviews·M PatarroyoC G Gahmberg
Aug 2, 1990·Nature·T A Springer
Apr 1, 1991·American Journal of Respiratory Cell and Molecular Biology·A ChambaD Burnett
Aug 14, 1987·Biochemical and Biophysical Research Communications·J A MarinoP J Spagnuolo
Sep 1, 1989·The Journal of Cell Biology·C NathanS D Wright
Nov 1, 1989·Experimental Cell Research·M KatayamaK Sekiguchi
May 1, 1989·The Journal of Cell Biology·H D GreshamE J Brown
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
May 1, 1988·The Journal of Clinical Investigation·Y T WachtfogelR W Colman
Jul 1, 1987·Reviews of Infectious Diseases·R A Proctor
Oct 1, 1988·The Journal of Surgical Research·D W EasterA N Ozkan
Apr 1, 1987·Cell Biochemistry and Function·W HornebeckL Robert
Oct 1, 1981·Cell·M D PierschbacherE Ruoslahti
Nov 1, 1995·The Journal of Cell Biology·C A OwenE J Campbell
May 1, 1995·Journal of Leukocyte Biology·D R Pakianathan
Dec 1, 1994·Journal of Leukocyte Biology·J A Smith
Aug 1, 1994·The Journal of Investigative Dermatology·F Grinnell, M Zhu
Oct 1, 1993·European Journal of Haematology·N BorregaardD F Bainton
Jun 1, 1993·Inflammation·P KubesD N Granger
Oct 1, 1995·Inflammation·J B Barna, R R Kew
Apr 12, 1996·Biochimica Et Biophysica Acta·O WiedowY Kameyoshi
May 1, 1996·The Journal of Experimental Medicine·T B IssekutzA C Issekutz
Jun 1, 1996·Journal of Leukocyte Biology·S M WahlJ B McCarthy
Dec 1, 1995·Seminars in Cell Biology·C A Owen, E J Campbell
Oct 1, 1996·The Journal of Experimental Medicine·T Q Cai, S D Wright
May 25, 1997·Experimental Cell Research·R SalcedoM Patarroyo
Citations
Aug 29, 2006·Hybridoma·Mariko Okada, Yasuo Hitsumoto
Dec 10, 1999·Experimental Cell Research·T GeberhiwotM Patarroyo
May 25, 1997·Experimental Cell Research·R SalcedoM Patarroyo
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brice KorkmazFrancis Gauthier
May 24, 2000·Thrombosis Research·A Bonnefoy, C Legrand