Endogenous GATA factors bind the core sequence of the tetO and influence gene regulation with the tetracycline system

Molecular Therapy : the Journal of the American Society of Gene Therapy
D J Gould, Y Chernajovsky

Abstract

The tetracycline-regulated eukaryotic gene expression systems have been applied in numerous areas of bioscience. The systems utilize a tetracycline-responsive promoter (P(tet)) and synthetic transactivators (tTA or rtTA) that bind to the promoter in the presence or absence of doxycycline, regulating gene expression. Both the basal activity of the P(tet) and the magnitude of regulation by the system vary between cell types. In this investigation we have mapped the positions of endogenous transcription factor binding sites within the P(tet) and through deletion studies determined the portion of the promoter that contributes to basal activity. The tetracycline operator (tetO) repeats appear to be the source of basal activity and they were shown to harbor motifs for GATA transcription factors. The GATA motif is located within the central core of the tetO and so has the potential to compete with tTA and rtTA binding. The molecular interactions of endogenous and overexpressed GATA factors with the GATA motif in the tetO were demonstrated and effects on function of the tetracycline-regulated gene expression system investigated. GATA factors are widespread in embryonic tissues, are expressed within several adult cell types, and display...Continue Reading

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Citations

Nov 13, 2008·Cytotechnology·Tobias MayDagmar Wirth
Nov 1, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Maria C SubangDavid Gould
Mar 30, 2013·Journal of Dental Research·C G WilsonR T Franceschi
Jan 9, 2010·European Journal of Social Psychology·Michelle N Shiota, Douglas T Kenrick
Mar 30, 2006·The Journal of Gene Medicine·Wilfried Weber, Martin Fussenegger
Mar 16, 2006·Psychological Bulletin·Serena ChenMolly Parker Tapias
Aug 2, 2017·Scientific Reports·Arslan AkmammedovRenato Paro

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