Endogenous glycogen prevents Ca2+ overload and hypercontracture in harp seal myocardial cells during simulated ischemia

Journal of Molecular and Cellular Cardiology
T HendenT S Larsen

Abstract

The purpose of this study was to determine if elevated myocardial glycogen content could obviate Ca(2+) overload and subsequent myocardial injury in the setting of low oxygen and diminished exogenous substrate supplies. Isolated harp seal cardiomyocytes, recognized as having large glycogen stores, were incubated under conditions simulating ischemia (oxygen and substrate deprivation) for 1 h. Rat cardiomyocytes were used for comparison. Freshly isolated seal cardiomyocytes contained approximately 10 times more glycogen than those from rats (479 +/- 39 vs. 48 +/- 5 nmol glucose/mg dry weight (dry wt), mean +/- S.E., n = 6), and during ischemia lactate production was significantly greater in seal compared to rat cardiomyocytes (660 +/- 99 vs. 97 +/- 14 nmol/mg dry wt), while glycogen content decreased both in seal (from 479 +/- 39 to 315 +/- 58 nmol glucose/mg dry wt) and rat cardiomyocytes (from 48 +/- 5 to 18 +/- 5 nmol glucose/mg dry wt). Cellular ATP was well maintained in ischemic seal cardiomyocytes, whereas it showed a 65% decline (from 31 +/- 3 to 11 +/- 1 nmol ATP/mg dry wt) in rat cardiomyocytes. Similarly, total seal cardiomyocyte Ca(2+) content was not affected by ischemia, while Ca(2+) increased from 8.5 +/- 2.0 to 13...Continue Reading

Citations

Oct 14, 2006·Annual Review of Physiology·Jan-Marino RamirezArnoldus S Blix
Mar 31, 2012·Circulation Research·Hannah V CareyDavid A Lathrop
Jul 31, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jessica U MeirPaul J Ponganis
Nov 28, 2019·Biology Letters·Chris G FaulkesDunja Aksentijevic
Aug 20, 2005·The Biochemical Journal·Giancarlo Solaini, David A Harris
Oct 4, 2007·American Journal of Physiology. Endocrinology and Metabolism·Patrick McConvilleRichard G Spencer
Jun 20, 2019·Frontiers in Physiology·Michael S Tift, Paul J Ponganis
Dec 7, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kelli J HarmonKenneth J Rodnick

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