Endogenous hydrogen peroxide increases biofilm formation by inducing exopolysaccharide production in Acinetobacter oleivorans DR1

Scientific Reports
In-Ae JangWoojun Park

Abstract

In this study, we investigated differentially expressed proteins in Acinetobacter oleivorans cells during planktonic and biofilm growth by using 2-dimensional gel electrophoresis combined with matrix-assisted laser desorption time-of-flight mass spectrometry. We focused on the role of oxidative stress resistance during biofilm formation using mutants defective in alkyl hydroperoxide reductase (AhpC) because its production in aged biofilms was enhanced compared to that in planktonic cells. Results obtained using an ahpC promoter-gfp reporter vector showed that aged biofilms expressed higher ahpC levels than planktonic cells at 48 h. However, at 24 h, ahpC expression was higher in planktonic cells than in biofilms. Deletion of ahpC led to a severe growth defect in rich media that was not observed in minimal media and promoted early biofilm formation through increased production of exopolysaccharide (EPS) and EPS gene expression. Increased endogenous H2O2 production in the ahpC mutant in rich media enhanced biofilm formation, and this enhancement was not observed in the presence of antioxidants. Exogenous addition of H2O2 promoted biofilm formation in wild type cells, which suggested that biofilm development is linked to defense a...Continue Reading

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Citations

Jan 19, 2018·Applied Microbiology and Biotechnology·Dipanjan SenguptaDipa Biswas
Dec 22, 2019·Applied Microbiology and Biotechnology·Bora ShinWoojun Park
Oct 28, 2017·The Journal of Microbiology·Bora Shin, Woojun Park
Mar 14, 2019·Scientific Reports·Manish KumarIndu Shekhar Thakur
Jan 5, 2020·Applied Microbiology and Biotechnology·Bora ShinWoojun Park
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fengyu GuoWeijun Chen
Jan 31, 2020·International Journal of Food Microbiology·Jinru Chen, Yin Wang
Oct 26, 2021·Journal of Bacteriology·Stefan Katharios-LanwermeyerGeorge A O'Toole

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Datasets Mentioned

BETA
GSE44347
GSE70356

Methods Mentioned

BETA
RNA-seq
PCR
scanning electron microscopy
electrophoresis

Software Mentioned

iCycler iQ
Flex Analysis
PDQuest
BamView
BLASTP
Artemis

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